Mkpụrụ dị elu T7 RNA Poymerase
Nkọwa
T7 RNA Polymerase dịpụrụ adịpụ na E.coli na-ebu plasmid nke nwere mkpụrụ ndụ T7 RNA Polymerase.T7 RNA Polymerase bụ RNA polymerase nke dabere na DNA nke akọwapụtara nke ọma maka ndị na-akwalite phage T7.
Ọdịdị Chemical
Nkọwa nkeji
A kọwapụtara otu nkeji dị ka ọnụọgụ nke enzyme nke ga-etinye 1 nmol ATP n'ime ihe anaghị edozi acid na mkpokọta mmeghachi omume nke 50 μl n'ime elekere 1 na 37 Celsius C na 1X RNA Polymerase Reaction Buffer.
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Ịdị ọcha nke Protein | ≥95% |
| Nickase | Ọnweghị nke achọpụtara |
| Rnase | Ọnweghị nke achọpụtara |
| Endonuclease | Ọnweghị nke achọpụtara |
| Exonuclease | Ọnweghị nke achọpụtara |
| Nkọwapụta nkwalite | Ngwaahịa enweghị nkọwa≤1.5% |
| E.coli gDNA | ≤0.1 pg/50U |
| Endotoxin | Nnwale LAL, ≤10EU/mg |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ikpụrụ kpọrọ nkụ
Nchekwa:Ekwesịrị ịchekwa ihe niile na -25 ℃ -15 ℃
Nnwale ndụ akwadoro:Afọ 2
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