Ihe mmachi BsaI Endonuclease egosipụtara

Mgbochi BsaI Endonuclease

Ịdị ọcha: 20U/μL

ngwugwu: 50μL, 250μL, 1ml, 10ml, 100ml

Zipu ajụjụ

Nkọwa ngwaahịa

Nkọwa

Enzyme mmachi BsaI bụ ụdị mmachi II endonuclease ewepụtara site na protein recombinant nke mkpụrụ ndụ BspQI debere na Bacillus sphaericus nke E.coli gosipụtara.A na-eji mmachi endonucleases eme ihe na eserese mkpụrụ ndụ ihe nketa, cloning na mpaghara ndị ọzọ.Enzyme a na-egbutu DNA ngwa ngwa maka mkpụrụ ndụ ihe nketa nke ọma Bsa I, ihe mgbochi IIs mgbochi endonuclease restriction endonuclease, sitere na nje E. coli recombinant nke na-ebu mkpụrụ ndụ BsaI nke cloned na gbanwee site na Bacillus stearothermophilus.nke nwere otu nkọwapụta dị ka enzymes nke ala na belata ọrụ kpakpando.Usoro njirimara ya na saịtị mkpochapụ bụ ndị a:

5'·······GGTCTC(N)··········3'

3'······CCAGAG(NNNN)········5'

Ọdịdị Chemical

Njirimara ngwaahịa

• Ihe mgbochi endonuclease nwere ọrụ enzymatic dị elu ma mejupụta mmeghachi omume mgbaze ngwa ngwa.

• Ọrụ endonuclease dị ala na-abụghị nke akọwapụtara iji hụ na igbubi "scalpel" ziri ezi.

• Enweghị usoro BSA, na-ebelata mmetọ isi iyi ọkụ.

Nkọwapụta

Nnwale ihe	Nkọwapụta
Enzyme ọrụ	20 U/µL
Ịdị Ọcha (SDS-PAGE)	≥95%
Rnase	Ọnweghị nke achọpụtara
Ọrụ DNA na-abụghị nke akọwapụtara	Ahụbeghị ya
Omume kpakpando	Ahụbeghị ya
Njikọ na mwepu	Mgbe mgbari gbazee, a ga-ekekọta iberibe DNA.Enwere ike iji Bsal gbutu iberibe irighiri ihe ndị a.
Endotoxins (LAL-nnwale)	≤10 EU/mg
E.Coli DNA	≤1 oyiri/2U

njikwa mma

Dị ọcha ≥ 95%, Ọ dịghị DNase, RNase ọrụ, Onye ọbịa DNA residue ≤ 100pg/mg, host protein residue ≤ -50ppm, endotoxin residue ≤ 10EU/mg, Enweghị protease ọrụ, sterile, free nke mycoplasma

Ngwa

Ghichaa plasmid iji dozie iberibe DNA nke nwere linearized na njedebe nke Poly (A/T/C/G);

Linearize plasmid template tupu in vitro ederede;

Mgbochi mgbari;

Nkọwa nkeji

A kọwapụtara otu nkeji dị ka ọnụọgụ enzyme achọrọ iji gbarie 1 μg nke DNA njikwa ime n'ime elekere 1 na 37 Celsius na mkpokọta mmeghachi omume nke 50 µL.