Mgbochi BspQI Endonuclease
Nkọwa
Enwere ike igosipụta BspQI n'otu n'otu na E. coli nke nwere ike ịmata saịtị ụfọdụ ma mepụta ya n'okpuru ụkpụrụ GMP.A na-eji ya na nkewa mkpụrụ ndụ ihe nketa, cloning na mpaghara ndị ọzọ.Enzyme a na-egbutu DNA ngwa ngwa maka usoro mkpụrụ ndụ ihe nketa nke ọma.
5'·······GGTCTC(N) 1··········3'
3'·······CCAGAG(N) 4·········5'
Ọdịdị Chemical
Nkọwa nkeji
A kọwapụtara otu nkeji dị ka ọnụọgụ enzyme achọrọ iji gbarie 1 µg nke λ DNA n'ime elekere 1 na 50 Celsius na mkpokọta mmeghachi omume nke 50 µl.
Njirimara ngwaahịa
• Ihe mgbochi endonuclease nwere nnukwu ọrụ enzymatic ma mejupụta mgbaze ngwa ngwa.
• Ọrụ endonuclease dị ala na-abụghị nke akọwapụtara iji hụ na igbubi "scalpel" ziri ezi.
• Enweghị usoro BSA, na-ebelata mmetọ isi iyi ọkụ.
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Ịdị ọcha nke Protein | 98% |
| Ihe fọdụrụ na protease | Ọnweghị nke achọpụtara |
| Nchọpụta njikọ na ịkpụ | Ịrụ mgbaze:95% |
| DNA | Ọnweghị nke achọpụtara |
| Nicaknase | Ọnweghị nke achọpụtara |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ebupu n'okpuru 0 Celsius
Nchekwa:Na-echekwa na -20 Celsius C
Nnwale ndụ akwadoro:Afọ 2
