T4 RNA ligase
Nkọwa
Ngwaahịa a bụ T4 RNA ligase (T4 RNA Ligase I) dabere na ATP nke E. coli gosipụtara.Enzyme a na-eme ka e guzobe njikọ phosphodiester n'etiti oligonucleotides, RNA nwere otu eriri na DNA intermolecular / intra-molecular 5'-PO4 na 3'-OH.
Nkọwa nkeji
A kọwapụtara otu nkeji dị ka ọnụọgụ enzyme achọrọ iji tụgharịa 1 nanomole nke 5′-[³²P] rA16 ka ọ bụrụ ụdị na-eguzogide phosphatase n'ime nkeji 30 na 37°C.
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Enzyme ọrụ | 10 U/μL |
| Ọrụ protein | ≥ 95% |
| Nickase | Enweghị ike ịhụ ya |
| Ọrụ RNase | Enweghị ike ịhụ ya |
| Ecoli gDNA | ≤1 oyiri/50U |
| Endotoxin | Nnwale LCL,≤10EU/mg |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ikpụrụ kpọrọ nkụ
Nchekwa:Chekwaa na -25 ~ -15ºC (zere ka oyi na-atụ ugboro ugboro na thawing)
Nnwale ndụ akwadoro:Afọ 2
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
