RT-LAMP Reagent (-20℃)
Nkọwa
A na-eji teknụzụ LAMP eme ihe ugbu a n'ọhịa nke isothermal amplification.Ọ na-eji 4-6 primers nke nwere ike ịchọpụta mpaghara 6 kpọmkwem na mkpụrụ ndụ ebumnuche, ma dabere na ọrụ mgbapụ strand siri ike nke Bst DNA polymerase.Enwere ọtụtụ ụzọ nchọpụta LAMP, gụnyere usoro agba agba, usoro pH colorimetric, usoro turbidity, HNB, calcein, wdg. RT-LAMP bụ otu ụdị mmeghachi omume LAMP nwere RNA dị ka ndebiri.Emepụtara Kit-LAMP Kit (UDG gbakwunyere) dabere na usoro fluorescence ma nwee Bst DNA Polymerase, transcriptase reverse thermotolerant, murine RNase inhibitor, na UDGase.Ngwa LAMP a nwere uche dị elu, ezigbo ngwa ngwa, yana arụmọrụ dị oke mma na nkọwapụta, nnabata na nkwụsi ike.
Ngwa
| 100T | 1000T |
| 600μL | 6ml |
| 100 μl | 1 ml |
1) Bst Buffer nwere dN(U)TP na SYTO-9, wdg, na 1 × Bst Buffer nwere 8 mmol/L Mg2+;
2) Bst Enzyme Mix (UDG gbakwunyere) nwere UDGase, Reverse Transcriptase, Bst Ⅱ Polymerase, MRI;
Ngwa
Mmeghachi omume LAMP
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -20 ℃
Ndụ Shief:Ọnwa isii
Ịdọ aka ná ntị
Maka nchekwa na ahụike gị, biko yiri uwe lab na gloves enwere ike iji rụọ ọrụ.
