Rnase inhibitor (glycerol n'efu)
Murine RNase inhibitor bụ ihe mgbochi murine RNase egosipụtara ma sachaa ya na E.coli.Ọ na-ejikọta na RNase A, B ma ọ bụ C na nha 1: 1 site na njikọta na-abụghị nke covalent, si otú a na-egbochi ọrụ nke enzymes atọ na ichebe RNA na mmebi.Agbanyeghị, ọ naghị adị irè megide RNase 1, RNase T1, S1 Nuclease, RNase H ma ọ bụ RNase sitere na Aspergillus.A nwalere inhibitor Murine RNase site na RT-PCR, RT-qPCR na IVT mRNA, yana dakọtara na azụmaahịa dị iche iche Reverse transcriptases, DNA polymerases na RNA polymerases.
Tụnyere ndị na-egbochi RNase mmadụ, murine RNase inhibitor enweghị cysteine abụọ nwere mmetụta dị ukwuu na oxidation nke na-akpata inactivation nke inhibitor.Nke ahụ na-eme ka ọ kwụsie ike na obere mkpokọta DTT (ihe na-erughị 1 mM).Njirimara a na-eme ka ọ dabara adaba maka mmeghachi omume ebe oke DTT dị njọ na mmeghachi omume (dịka RT-PCR oge).
Angwa
Enwere ike iji ngwaahịa a mee ihe na nnwale ọ bụla ebe nnyonye anya RNase ga-ekwe omume iji zere mmebi RNA, dị ka:
1.First-strand cDNA njikọ, RT-PCR, RT-qPCR, wdg;
2.Chebe RNA site na nbibi na in-vitro transcript / ntụgharị (dịka ọmụmaatụ viral replication in vitro);
3.Mgbochi nke ọrụ RNase n'oge RNA kewapụrụ na ịdị ọcha.
Ọnọdụ Nchekwa
Na-echekwa na -25～-15 ℃;
Oyiyi na-agbaze oyi ≤ 5 ugboro;
Kwesịrị ekwesị maka afọ 1.
Nkọwa nkeji
A kọwapụtara otu nkeji dị ka ọnụọgụ RNase Inhibitor chọrọ iji gbochie ọrụ 5 ng nke RNase A site na 50%.
Ibu molekụla
RNase Inhibitor (Glycerol-free) bụ protein 50 kDa.
njikwa mma
Exonuclease Ihe omume:
Ntinye nke 40 U nke enzyme na 1 μg λ-Hind III na-agbari DNA maka awa 16 na 37 Celsius C arụpụtaghị mmebi nke DNA nke nwere ike ịchọpụta dị ka gel electrophoresis kpebisiri ike.
Ọrụ Endonuclease:
Ntinye nke 40 U nke enzyme na 1 μg λ DNA maka awa 16 na 37 ° C emeghị ka DNA ghara ịchọta DNA dị ka gel electrophoresis kpebisiri ike.
Ịkpọ oku Ihe omume:
Ntinye nke 40 U nke enzyme na 1 μg pBR322 maka awa 16 na 37 ℃ arụpụtaghị mmebi nke DNA nke nwere ike ịchọpụta dị ka gel electrophoresis kpebisiri ike.
RNase Ihe omume:
Ntinye nke 40 U nke enzyme na 1.6 μg MS2 RNA maka awa 4 na 37 ℃ arụpụtaghị mmebi nke RNA a na-achọpụta dị ka gel electrophoresis kpebisiri ike.
E.coli DNA:
Achọpụtara 40 U nke Enzyme site na TaqMan qPCR.DNA E.coli bụ ≤ 0. 1pg/40U.
Notes
1.Adịla ịma jijiji ma ọ bụ kpalie ike iji gbochie inactivation enzyme.
2.RNase inhibitor na-arụ ọrụ na okpomọkụ sitere na 25 ℃ ruo 55 ℃ ma na-adịghị arụ ọrụ na ≥65 ℃.
3. A naghị egbochi ọrụ RNase H, RNase 1 na RNase T1.
4.The pH nso maka igbochi ọrụ RNase dị obosara (na-arụ ọrụ na pH 5-9), na-egosipụta ọrụ kachasị na pH 7-8.
