Reagent Mwepụta Sample
Sample Release Reagent bụ maka ọnọdụ nyocha nchọpụta POCT.Maka sistemu abụọ nke ịgbatị LAMP ozugbo na PCR na-abawanye, ọ dịghị mkpa maka mmịpụta acid nucleic.Enwere ike ịbawanye lysate crude nke ihe nlele ahụ, enwere ike ịchọpụta mkpụrụ ndụ ebumnuche nke ọma, enwere ike belata oge nchọpụta ihe nlele, nke dabara nke ọma ngwa chọrọ nke POCT molekul.Ọ dị mma maka imi imi, akpịrị akpịrị na ụdị ihe atụ ndị ọzọ.Enwere ike iji ihe nlele ndị ahụ edoziri ozugbo maka nchọpụta oge fluorescence quantitative PCR ma ọ bụ LAMP, enwere ike nweta otu nsonaazụ ahụ dị ka ụzọ mmịpụta ọdịnala na-enweghị ọrụ mmịpụta nucleic acid siri ike.
Ọnọdụ Nchekwa
Ụgbọ njem na ụlọ ahịa na ụlọ okpomọkụ.
njikwa mma
Nchọpụta arụrụ arụ – quantitative qPCR: 800μl Sample Release Reagent system abawanyela.
ya na 1000 nobi novel Pseudovirus, otu imi imi imi, na-arụpụta akụkụ mgbawa yiri nke ahụ naΔCt ụkpụrụ n'ime ± 0.5 Ct.
Usoro nnwaleres
1. Were 800 μl Sample Release Reagent wee gbasaa ngwọta lysis n'ime tube nlele 1.5 ml
2. Were imi imi ma ọ bụ akpịrị akpịrị na swab; Usoro nlele imi: were swab na-adịghị mma ma tinye ya n'oghere imi, jiri nwayọọ nwayọọ na-aga n'ihu ruo ihe dịka 1.5 cm miri, jiri nwayọọ gbanwee ugboro 4 megide mucosa imi maka ihe karịrị 15 sekọnd. , wee megharịa otu ọrụ ahụ na oghere imi nke ọzọ na otu swab. Usoro nyocha nke akpịrị akpịrị: were swab na-adịghị mma ma jiri nwayọọ nwayọọ hichaa tonsils pharyngeal na azụ pharyngeal mgbidi ugboro 3.
3.Tinye swab ozugbo na tube nlele.Ekwesịrị ịtụgharị isi swab ahụ ma gwakọta ya na ngwọta nchekwa ma ọ dịkarịa ala 30 sekọnd iji hụ na ihe nlele ahụ zuru oke na tube nlele.
4. Incubation na ụlọ okpomọkụ (20 ~ 25 ℃) maka 1min, a na-emecha nkwadebe nke lysis buffer.
5. Ma 25μl usoro RT-PCR na RT-LAMP dakọtara na 10μl ego nke template mgbakwunye maka nchọpụta nchọpụta.
Ihe ndetu
1. Opekempe kacha nta nke sample lysate kpọmkwem kwekọrọ na otu swab nwere ike gbanwee na 400μl, nke nwere ike gbanwee dị ka mkpa ule.
2. Ozugbo sample esichara site sample ntọhapụ reagent, ọ na-atụ aro na-eduzi na-esonụ na-adọ nke ule ozugbo o kwere omume, na etiti oge nchere oge bụ ọkachamma ihe na-erughị 1 awa.
3. pH nke lysate sample bụ acidic, na usoro nchọpụta ahụ kwesịrị inwe ihe nchekwa ụfọdụ.Ọ dabara maka ọtụtụ PCR, RT-PCR, na LAMP nchọpụta fluorescence na pH buffer, mana ọ dabaraghị maka nchọpụta LAMP colorimetric na-enweghị ihe nchekwa.
