Ngwa nyocha Multiplex Otu Nzọụkwụ RT-qPCR (UDG Plus)
Nkọwa
Multiplex One Step RT-qPCR Kit (UDG Plus) bụ ngwa nyocha ngwa ngwa RT-qPCR dabere na nyocha nke dabara maka PCR otu-plex ma ọ bụ multi-plex quantitative na-eji RNA dị ka ndebiri (dị ka nje RNA).A na-eji ya na-amụba DNA nke nwere eriri abụọ site na ndebiri RNA nwere eriri iji mee ka ọnụọgụ RNA dị ngwa ngwa ozugbo.N'ime ntụgharị ntụgharị ntụgharị ntụgharị ntụgharị transcriptase na-ejikọta mkpụrụ ndụ DNA nke nwere otu stranded (cDNA) dabara na ndebiri RNA.
Usoro mmeghachi omume
Ngwa
| Ngwa | 200rxn | 1,000rns | 10,000 rxn |
| ddH2O na-enweghị RNase | 3 * 1 ml | 15ml | 150ml |
| 5 * otu nzọụkwụ mix | 800μl | 4 * 1 ml | 40ml |
| Otu nzọụkwụ enzyme mix | 200μl | 1 ml | 10ml |
| 50* ROX ntụaka agba agba 1 | 80μl | 400μl | 4 * 1 ml |
| 50* ROX ntụaka agba agba 2 | 80μl | 400μl | 4 * 1 ml |
a.Nchekwa otu nzọụkwụ gụnyere dNTP Mix na Mg2+.
b.Ngwakọta Enzyme na-enwekarị transcriptase reverse, Hot Start Taq DNA polymerase (mgbanwe mgbochi mmadụ) na RNase inhibitor.
c.Ejiri ya iji dozie njehie nke sinal fluorescene n'etiti olulu mmiri dị iche iche.
c.ROX: Ịkwesịrị ịhọrọ nhazi n'ụdị ngwaọrụ nnwale.
Ngwa
Nchọpụta nke pathogens
nchoputa na nyocha nke etuto
Nchọpụta ọrịa anụmanụ
Nchọpụta mmalite nke ọrịa nke eketa
Nchọpụta nke microorganisms pathogenic na nri
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -30 ~ -15 ℃.
Ndụ Shief:1 afọ
