Deoxyribonuclease I recombinant (DNase I, RNase enweghị)
Nkọwa
Recombinant Deoxyribonuclease I, bụ endonuclease nke nwere ike igbari DNA nwere otu eriri ma ọ bụ nke nwere okpukpu abụọ.Ọ na-eme njikọ phosphodiester iji mepụta mono- na oligodeoxynucleotides nwere otu 5'-phosphate na otu 3'-OH.Oke pH kacha arụ ọrụ nke DNAse I bụ 7-8.Ọrụ nke DNase I dabere na Ca2 + ma nwee ike rụọ ọrụ site na ion metal divalent dị ka Co2 +, Mn2 +, Zn2 +, wdg N'ihu Mg2 +, DNA m nwere ike belata DNA abụọ na-enweghị usoro na saịtị ọ bụla;mgbe ọ nọ n'ihu Mn2+, DNase m nwere ike ibelata DNA nke nwere eriri abụọ n'otu saịtị ahụ, na-eme ka njedebe na-adịghị mma ma ọ bụ 1-2 nuclei Sticky ends na nucleotide overhangs.A na-enweta enzyme a site na recombinant E. coli strains, enweghị RNase, a pụkwara iji ya mee ihe maka nhazi nke ụdị RNA dị iche iche.
Ọdịdị chemical
Ngwa
Mwepụta RNA: kwadebe RNA na-enweghị DNA;
Mgbe transcript in vitro na RNA polymerase, dị ka T7 RNA Polymerase (Cat # 10618), a na-eji wepụ DNA template;
Kwadebe RNA na-enweghị DNA tupu RT-PCR na RT-qPCR;
Ejiri ya na DNA Polymerase I (Cat#12903) maka ịkpọ DNA site na ntụgharị nick;
Ejiri ya na nyocha akara ukwu iji nyochaa mmekọrịta DNA-protein;
Mepụta ọbá akwụkwọ mpempe akwụkwọ na-enweghị usoro;
Na nchọpụta TUNEL nke apoptosis, a na-eji DNA genomic nke a kpachapụrụ anya mee ihe dị ka njikwa dị mma.
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -20 ℃
Ndụ nchekwa:afọ 2
ndetu:
1.Ekwesịrị ịchekwa enzymes n'ime igbe ice ma ọ bụ na mmiri ịsa mmiri mgbe ejiri ya, a ga-echekwa ya na -20 Celsius C ozugbo ejiri ya.
2.Maka nchekwa gị na ahụike gị, biko yie ihe nchekwa nkeonwe (PPE), dị ka uwe ụlọ nyocha na uwe mwụfu, mgbe ị na-eji ngwaahịa a arụ ọrụ.
