Uracil DNA Glycoylase (enweghị Glycerol)
Nkọwa
Thermosensitive UDG (uracil-DNA glycosylase) nwere ike kpalite hydrolysis nke uracil isi nke DNA yinye nwere uracil na N-glycosidic njikọ nke sugar-phosphate azụ ọkpụkpụ ka hapụ free uracil.N'iji ya na enzymes UDG nkịtị, enzymes UDG na-ekpo ọkụ na-ezere ọrụ fọdụrụnụ nke UDG enzymes mgbe arụghị ọrụ, nke nwere ike imebi ngwaahịa nkwalite dU nwere n'ime ụlọ okpomọkụ.Ngwaahịa a na-arụ ọrụ na ọnụ ụlọ okpomọkụ ma na-enwe mmetụta okpomọkụ na ọ na-adịkarị mfe ịmalite.
Ọdịdị Chemical
Nkọwapụta
| Enzyme | Glycosylase |
| Ihe nchekwa dakọtara | Nchekwa nchekwa |
| Ọkụ anaghị arụ ọrụ | 50°C, 10 nkeji |
| Nkọwa nkeji | A kọwapụtara otu nkeji (U) dị ka ọnụọgụ enzyme achọrọ iji kpata hydrolysis nke 1 μg dU nwere dsDNA n'ime nkeji 30 na 25°C. |
Ngwa
Wepu mmetọ ikuku ikuku PCR nwere dU.
Mwepụ ntọala uracil site na DNA nwere otu eriri ma ọ bụ nke nwere okpukpu abụọ
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -15 ℃ ~ -25 ℃
Ndụ Shief:1 afọ
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
