Ngwa nyocha DNA (Fluorescence)

Nọmba Cas: 9003-98-9

Ịdị ọcha: 90%

Ngwugwu: 48Nnwale, 192Nnwale

Zipu ajụjụ

Nkọwa ngwaahịa

Nkọwa

Ngwa nchọpụta DNA dabere na nyocha DNA nke akpọrọ fluorophore.Mgbe ihe nlele ahụ enweghị ọrụ DNA, nyocha ahụ kwụsiri ike ma ọ naghị emepụta mgbama fluorescent;mgbe ihe nlele ahụ nwere ọrụ DNase, nyocha ahụ na-eweda ya ala, na-ebute mgbama fluorescence ji nwayọọ nwayọọ na-abawanye;Ọnụ ọgụgụ nke mmụba nke mgbaàmà fluorescence na-ejikọta nke ọma na ọnụọgụ na ọrụ nke enzymes.Jiri ihe na-agụ microplate fluorescence iji tụọ n'ogo nke ex/em=485/525nm iji chọpụta ma DNA emeruwo ihe nlele ahụ.

Ọdịdị Chemical

Ngwa mejupụtara

Aha	48Nnwale	192Nnwale
10× mmeghachi omume ngwọta	0.5ml	2.0ml
Nyocha DNA	1 tube	1 tube
Ihe nchekwa TE	0.5ml	2.0ml
Ọkọlọtọ DNAse I (2U/μL)	10μL	20μL
Ọkọlọtọ Dilution ihe nchekwa	6ml	12ml
Mmiri DNA na-enweghị RNase	25ml	25ml
DNAse RNase pụọ	50ml	50ml

Nkọwapụta

Nnwale ihe	Nkọwapụta
Ọdịdị	Nkwakọ ngwaahịa zuru oke na enweghị mmiri mmiri
Oke nchọpụta	DNA I: 1.25x10^-6U/μL
Nkenkenke	Intra assay CV ≤ 10%