Ngwa nyocha nke RNase (Fluorescence)
Nkọwa
Ngwa nchọpụta RNase dabere na nyocha RNA nke akpọrọ fluorophore, na-enyere ndị nyocha aka tụọ ọrụ RNase.Mgbe ihe nlele ahụ enweghị ọrụ RNase, nyocha ahụ kwụsiri ike ma ọ naghị emepụta mgbama fluorescent;mgbe ihe nlele ahụ nwere ọrụ RNase, nyocha ahụ na-eweda ya ala, na-ebute mgbama fluorescence ji nwayọọ nwayọọ na-abawanye;Ọnụego mmụba nke mgbaàmà fluorescence na-ejikọta nke ọma na ọnụọgụ na ọrụ nke enzymes.
Ọdịdị Chemical
Ngwa mejupụtara
| Aha | 48Nnwale | 192Nnwale |
| 10× mmeghachi omume ngwọta | 0.5ml | 2.0ml |
| nyocha RNA | 1 tube | 1 tube |
| Ihe nchekwa TE | 0.5ml | 2.0ml |
| RNase A ọkọlọtọ (10mg/ml) | 10μL | 20μL |
| Ọkọlọtọ Dilution ihe nchekwa | 6ml | 12ml |
| Mmiri DNA na-enweghị RNase | 25ml | 25ml |
| DNAse RNase pụọ | 50ml | 50ml |
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Ọdịdị | Nkwakọ ngwaahịa zuru oke na enweghị mmiri mmiri |
| Oke nchọpụta | RNase A: 0.313pg/mL |
| Nkenkenke | Intra assay CV ≤ 10% |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ikpụrụ kpọrọ nkụ
Nchekwa:Chekwaa na -20C (zere ka oyi na-atụ ugboro ugboro na thawing)
Nnwale ndụ akwadoro:1 afọ
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
