Trypsin recombinant
Uru
1.Ọ dịghị anụmanụ si malite
2.High ịdị ọcha
3.Better njikwa usoro
4.Stable àgwà
Nkọwa
Trypsin na-agbaji kpọmkwem C-terminal peptide bonds nke lysine na arginine, nke nwere ike imebi protein na-ejikọta intercellular.Usoro amino acid nke recombinant trypsin nke Hzymes Biotech na-emepụta bụ otu ihe ahụ nke trypsin nke porcine sitere na pancreas, ma na-emepụta ya site na nkwupụta Escherichia coli recombinant.Ọ nwere ike dochie mmịpụta ọdịnala nke trypsin maka mgbari sel na ọgwụ mgbochi, mkpụrụ ndụ stem, ọgwụgwọ sel mgbochi, nyocha ọgwụ, ọgwụ mgbochi na mpaghara ndị ọzọ.Aprotinin, soybean trypsin inhibitor, wdg nwere ike igbochi ọrụ trypsin nke ọma.
trypsin recombinant nwere otu ihe eji eme ka trypsin ewepụtara na okike.Emepụtara ngwaahịa a n'ụlọ ọrụ ọkwa GMP.A naghị eji akụrụngwa sitere n'anụmanụ mee ihe na gbaa ụka, ịdị ọcha na ihe ikpeazụ.N'otu oge ahụ, a kwadoro usoro mmepụta na njikwa mma, na-eme ka ọkwa dị elu dị n'etiti batches.
Ọdịdị Chemical
Nkọwa nkeji
25 ℃, pH 7.6, 3.2ml mmeghachi omume ngwọta (1cm ìhè ụzọ), otu trypsin unit (U) kọwara dị ka ihe
mmụba nke 0.003 na uru absorption na 253nm site na enzymatic hydrolysis nke BAEE kwa nkeji.
Iji ngwaahịa
Maka omenala cell
• Tissue block mgbaze, isi cell nnweta.
• Na-agafe mgbaze nke sel ndị na-akwado ya.
• Omenala cell site na usoro microcarrier.
• Na-agbari mkpụrụ ndụ stent nke ọma.
• Usoro ọgwụgwọ mgbochi ọrịa, wdg.
Maka protein recombinant
• mmepụta insulin recombinant.
• Usoro nhazi nke protein, nkewa peptide.
• Usoro proteolytic akọwapụtara dị ka nyocha proteomics
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Ọdịdị | White ma ọ bụ gbanyụọ ọcha ntụ ntụ |
| Solubility (10mg/ml) | Kpochapụ ngwọta enweghị agba |
| Ịdị ọcha | Β-trypsin ≥70% α-trypsin ≤ 20% |
| Ihe omume akọwapụtara (ntụ ntụ) | ≥2500U/mg |
| E.Coli host protein fọdụrụnụ | ≤0.01% |
| Endotoxins (LAL-Nnwale) | ≤20 EU/mg |
Ụgbọ njem na nchekwa
Ụgbọ njem:A na-ebufe ya n'okpuru 2-8 Celsius
Nchekwa:Chekwaa na -2-8°C, mgbe agbazere ya na 1mM hcl ma ọ bụ 50nM HAC, kwesịrị ịchekwa n'okpuru -25 ~ -15 Celsius.
Nnwale ndụ akwadoro:Afọ 2
