Ultra Nuclease

Nkesa Nọmba: 9025-65-4

EC.Nọmba: 3.1.30.2

Ngwugwu: 20μL, 200μL, 2ml, 20ml

Zipu ajụjụ

Nkọwa ngwaahịa

Nkọwa

UltraNuclease bụ endonuclaese mebere site na mkpụrụ ndụ ihe nketa nke sitere na Serratia marcescens, nke nwere ike imebi DNA ma ọ bụ RNA, ma ọ bụ okpukpu abụọ ma ọ bụ otu eriri, linear ma ọ bụ okirikiri n'okpuru ọnọdụ dịgasị iche iche, na-eweda nucleic acid kpamkpam n'ime 5'-monophosphate oligonucleotides na 3-5. ogologo isi.

Mgbe mgbanwe mgbanwe mkpụrụ ndụ ihe nketa, ngwaahịa ahụ fermented, kwupụta, ma dị ọcha na Escherichia coli (E. coli), nke na-ebelata viscosity nke cell supernatant na cell lysate na nchọpụta sayensị, ma na-emeziwanye arụmọrụ nke ịdị ọcha na nyocha ọrụ nke protein.Enwere ike iji ya na usoro ọgwụgwọ mkpụrụ ndụ ihe nketa, nje virus, mmepụta ọgwụ mgbochi, protein na polysaccharide ụlọ ọrụ na-emepụta ọgwụ dị ka ihe na-ewepụ ihe reagent nucleic acid.

Ọdịdị Chemical

Nkọwa nkeji

Ọnụ ego nke enzyme eji agbanwe uru absorption nke △A260 site na 1.0 n'ime 30min na 37 Celsius C, pH 8.0, dakọtara na 37μg salmon sperm DNA gbarie site n'ịba n'ime oligonucleotides, akọwara dị ka ihe na-arụ ọrụ.

Ojiji na usoro onunu ogwu

• Wepụ exogenous nucleic acid na ngwaahịa ọgwụ mgbochi, belata ihe ize ndụ nke fọdụrụ n'ime nucleic acid toxicity na melite ngwaahịa nchekwa.
• Belata viscosity nke mmiri mmiri nri nke nucleic acid kpatara, belata oge nhazi na ịbawanye mkpụrụ protein.
• Wepu nucleic acid nke ọbọp urughuru (nje Virus, nsonye ahu, wdg), nke na-eme ka ntọhapụ na ọcha nke urughuru.
• Ọgwụgwọ nuklia nwere ike imezi mkpebi na mgbake nke nlele maka chromatography kọlụm, electrophoresis na nyocha blotting.
• Na usoro ọgwụgwọ mkpụrụ ndụ ihe nketa, a na-ewepụ acid nucleic iji nweta nje ndị metụtara adeno dị ọcha.

Nkọwapụta

Nnwale ihe	Nkọwapụta
Nkọwa	Dị ọcha na enweghị agba
Ihe omume	≥ 250 U/ul
Ọrụ akọwapụtara	≥1.1*10⁶U/mg
Ịdị ọcha (SDS-PAGE)	≥ 99.0%
Protease	Ọnweghị nke achọpụtara
Ibu ibu	10 cfu/100,000U
Endotoxines (LAL-nnwale)	0.25EU/1,000U