Alkaline Phosphatase (ALP)

Alkaline Phosphatase sitere na nje E. coli recombinant nke na-ebu mkpụrụ ndụ TAB5.Enzyme ahụ na-akwalite dephosphorylation nke 5' na 3' nsọtụ DNA na RNA phosphomonoesters.Ọzọkwa, ọ hydrolyses ribose, yana deoxyribonucleoside triphosphates (NTPs na dNTPs).TAB5 Alkaline Phosphatase na-arụ ọrụ na 5′ na-apụta, 5′ agbahapụla na njedebe.Enwere ike iji Phosphatase n'ọtụtụ ngwa usoro ihe ọmụmụ ihe, dị ka cloning ma ọ bụ akara njedebe nyocha iji wepụ nsọtụ phosphorylated nke DNA ma ọ bụ RNA.N'ime nyocha nke cloning, dephosphorylation na-egbochi DNA plasmid linearized site na njide onwe onye.Ọ nwekwara ike weda dNTP enweghị njikọ na nzaghachi PCR iji kwado ndebiri maka usoro DNA.Enzyme ahụ kpamkpam na enweghị ike ịgbagha ya site na ikpo ọkụ na 70 Celsius C maka nkeji 5, si otú ahụ na-eme ka mwepụ nke phosphatase dị tupu ligation ma ọ bụ njedebe na-enweghị isi.