RT-LAMP Fluorescent (bọọlụ Lyophilized)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) nwere ihe nkpuchi mmeghachi omume, Bst DNA polymerase na transcriptase na-eguzogide okpomọkụ, ihe nchebe lyophilized, na agba fluorescent.Ngwaahịa ahụ dị n'ụdị beads lyophilized, na naanị primers na ndebiri ka a ga-agbakwunye maka ojiji.
Ngwakọta reagent
RT-LAMP Fluorescent Master Mix (Lyophilized ntụ ntụ)
Ojiji
Maka DNA ma ọ bụ RNA isothermal amplification
Ọnọdụ Nchekwa
Ụgbọ njem na ụlọ ahịa na 2 ~ 8 ° C.
Ndụ nchekwa: ọnwa iri na abụọ.
Protocol
1. Wepụ ọnụ ọgụgụ nke lyophilized beads dị ka ọnụọgụ ule si dị.
2. Nkwadebe usoro mmeghachi omume
| Akụkụ | Usoro onunu ogwu |
| RT-LAMP Fluorescent Master Mix (Lyophilized ntụ ntụ) | 2 beads |
| Ọcha fluorescent | x μL |
| 10 × Ngwakọta Ngwakọta a | 5 μl |
| Ụdị DNA/RNA b | n μl |
| Mkpokọta | 50 μl |
ndetu:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.A na-atụ aro template nucleic acid ka agbaze na mmiri DEPC.
Ụgwọ nke ụlọ ọrụ Hyasen Biotechnology Co., Ltd
3) mmemme mmeghachi omume
| Okpomọkụ | Oge | okirikiri |
| 65 ℃ | 60s a | 30 |
ndetu:
1. Anakọta akara fluorescent
Ihe ndetu
1. Enwere ike ịkwalite okpomọkụ mmeghachi omume n'etiti 62 ° C na 68 ° C dị ka primer si dị.ọnọdụ;
2. Nnwale ahụ kwesịrị ịhazi ọrụ ahụ, gụnyere nkwadebe nke usoro mmeghachi omume,nhazi sample na dosing;
3. Iji zere mmetọ, a na-atụ aro ka ịkwadebe usoro mmeghachi omume na tebụl dị ọchama tinye ndebiri na mkpuchi uzuzu n'ime ụlọ ndị ọzọ ka ịzenarị ndabichi dị mma.
