dATP/dCTP/dGTP/dTTP/dUTP (100mM)
Nkọwa
Ngwaahịa a bụ mmiri mmiri na-enweghị agba.Ọ dabara maka nnwale dị iche iche nke mkpụrụ ndụ ihe omimi dị ka mmụba PCR, PCR ozugbo, cDNA ma ọ bụ njikọ DNA nkịtị, usoro DNA na ntinye aha.Enwere ike ịgbanye ya na mmiri dị ọcha, ma gbanwee ya na pH 7.0 na ngwọta NaOH dị elu, na ịdị ọcha ≥ 99% (HPLC).Mgbe achọpụtara ya, o nweghị DNAse, RNase na phosphotase.Enwere ike iji ya mee ihe ozugbo na mmeghachi omume nje dị iche iche dị ka PCR.
Ọdịdị chemical
Nkọwapụta
| Nwalee ihe | Nkọwapụta |
| Ọdịdị | Kpochapụ ngwọta enweghị agba |
| Ntinye uche | 100mmM ± 3% |
| Ịdị ọcha (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Njikwa ogo1. Nke a nkwadebe bụ free nke DNAse na RNase ofufe 2. A nwalere ọtụtụ dNTP a na arụmọrụ Taq DNA polymerase na Pfu DNA polymerase Nke a nke d NTP nwere arụmọrụ nwalere na Q-PCR | |
Ngwa
Ogologo ogologo PCR (20kb)
cDNA njikọ na RT-PCR
PCR oge ochie
PCR ọkọlọtọ
PCR zuru oke
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -25 ～-15 ℃
Ụbọchị nlele ọzọ akwadoro:afọ 2
Ihe ndetu
1) Enwere ike igbari ya na ụlọ okpomọkụ.Mgbe mgbasa, a ga-echekwa ya na igbe ice ma ọ bụ mmiri ịsa ahụ.Mgbe ejiri ya, a ga-echekwa ya na -25～-15 ℃ ozugbo.
2) Maka nchekwa gị na ahụike gị, biko yie mkpuchi ụlọ nyocha na gloves enwere ike iji rụọ ọrụ.
