Ribonuclease A (RNase A)

Ribonuclease A (RNase A) bụ polypeptide nwere otu eriri nwere njikọ disulfide 4 nwere ịdị arọ molekụla nke ihe dịka 13.7 kDa.RNase A bụ endoribonuclease nke na-eweda RNA nwere otu eriri na ihe fọdụrụ C na U.Kpọmkwem, mgbawa ahụ na-amata njikọ nke phosphodiester nke 5'-ribose nke nucleotide na otu phosphate na-emepụta na 3'-ribose nke nucleotide pyrimidine dị n'akụkụ, nke mere na 2', 3' - Cyclic phosphates bụ hydrolyzed na kwekọrọ ekwekọ. 3'-nucleoside phosphates (dịka ọmụmaatụ, pG-pG-pC-pA-pG bụ RNase A gbawara iji mepụta pG-pG-pCp na A-PG).RNase A bụ onye kacha arụ ọrụ na nkewa RNA nwere eriri ma na-arụ ọrụ n'ụdị mmeghachi omume dị iche iche: na obere nnu nnu (0 ruo 100 mM NaCl), enwere ike iji ya kewaa RNA nwere eriri, RNA nwere eriri abụọ, na RNA strands na RNA-DNA ngwakọ.ebe ọ nọ n'ọkwa nnu dị elu (≥0.3 M), RNase A nwere ike kewaa RNA nwere otu eriri.