Cholesterol oxidase (COD/CHOD)
Nkọwa
Cholesterol oxidase (CHOD) na-eme ka nzọụkwụ mbụ na catabolism cholesterol.Ụfọdụ nje bacteria na-abụghị pathogenic, dị ka Streptomyces na-enwe ike iji cholesterol dị ka isi iyi carbon.Nje bacteria na-akpata ọrịa, dị ka Rhodococcus equi, chọrọ CHOD ka ọ bute macrophage nke onye ọbịa. CHOD bụ ihe na-arụ ọrụ. Cholesterol na-ebu ụzọ oxidized na cholest-5-en-3-one na nzọụkwụ FAD chọrọ.Cholest-5-en-3-one bụ isomerized ka ọ bụrụ cholest-4-en3-one. Mmeghachi omume isomerization nwere ike gbanwee akụkụ ụfọdụ.Ọrụ CHOD dabere na njirimara anụ ahụ nke akpụkpọ ahụ nke ejikọtara mkpụrụ.
A na-eji CHOD chọpụta cholesterol ọbara.Ọ bụ enzyme nke abụọ a na-ejikarị eme ihe na ngwa nyocha mgbe glucose oxidase gasịrị.CHOD na-ahụkwa ngwa na microanalysis nke steroid na nri nri na n'ichepụta 3-ketosteroids site na 3b-hydroxysteroids. A na-enyocha osisi transgenic na-egosipụta cholesterol oxidase n'ọgụ megide owu boll weevil.A na-ejikwa kọlesterol oxidase mee ihe dị ka ihe nyocha nke mkpụrụ ndụ iji mee ka ọ pụta ìhè n'ụdị akpụkpọ ahụ cellular.
Ọdịdị Chemical
Ụkpụrụ mmeghachi omume
Cholesterol + O2 →△4-Cholesten-3-otu + H2O2
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | ntụ ntụ amorphous odo, lyophilized |
| Ihe omume | ≥8U/mg |
| Ịdị ọcha (SDS-PAGE) | ≥90% |
| Solubility (10mg ntụ ntụ / ml) | Kpochapụ |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ebufere n'okpuru -15 Celsius C
Nchekwa:Na-echekwa na -25~-15°C(Ogologo oge), 2-8°C(Obere oge)
Nnwale akwadoroNdụ:1 afọ
