Glucose Dehydrogenase (GDH)
Nkọwa
Glutamate Dehydrogenase (GDH) bụ mitochondrial enzyme nke na-eme ka deamination oxidative reversible nke glutamate na a-ketoglutarate ma na-eje ozi dị ka isi njikọ n'etiti anabolic na catabolic ụzọ.N'ime anụ mammals, GDH nọ n'okpuru ụkpụrụ allosteric ma nwee nnukwu ọrụ na imeju, akụrụ, ụbụrụ, na pancreas.Enwere ike iji ọrụ GDH na serum mee ka ọdịiche dị n'etiti ọrịa imeju n'ihi nsị imeju, nke na-egosighi ọrụ GDH serum dị elu, na ọrịa ndị na-ebute hepatocyte necrosis, nke na-eme ka ọbara GDH dị elu.
A na-ekpebi ọrụ GDH site na nyocha enzyme jikọtara ọnụ nke GDH na-emepụta NADH na-eri glutamate, nke na-emeghachi omume na nyocha na-emepụta ngwaahịa colorimetric (450 nm) dabara na ọrụ GDH dị ugbu a.Otu nkeji GDH bụ ọnụọgụ enzyme nke ga-emepụta 1.0 mmole nke NADH kwa nkeji na pH 7.6 na 37 Celsius C.
Ọdịdị Chemical
Usoro mmeghachi omume
D-glucose + onye na-anabata → D-glucono-1,5-lactone + belatara onye na-anabata ya.
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | Ọcha amorphous ntụ ntụ, lyophilized |
| Ihe omume | ≥160U/mg |
| Ịdị Ọcha (SDS-PAGE) | ≥90% |
| Solubility (10 mg ntụ ntụ / ml) | Kpochapụ |
| Enzymes na-emerụ ahụ | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Ụgbọ njem na nchekwa
Ụgbọ njem: Ngwunye ice
Nchekwa:Na-echekwa na -25 ~ -15°C (Ogologo oge), 2-8°C (obere oge)
Nnwale akwadoroNdụ: Ọnwa iri na asatọ
