Fructosyl-peptide oxidase (FPOX)
Nkọwa
Enzyme ahụ bara uru maka mkpebi nke fructosyl-peptide na fructosyl-L-amino acid.
Ọdịdị Chemical
Ụkpụrụ mmeghachi omume
Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | Ọcha amorphous ntụ ntụ, lyophilized |
| Ihe omume | ≥4U/mg |
| Ịdị Ọcha (SDS-PAGE) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Ụgbọ njem na nchekwa
Ụgbọ njem: Ambient
Nchekwa:Na-echekwa na -20°C (Ogologo oge), 2-8°C (obere oge)
Nnwale akwadoroNdụ:Afọ 2
Akụkọ mmepe
Otu n'ime ndenye aha eji achọpụta ọrịa shuga bụ haemoglobin glycated (HbA1c).Ntụle HbA1c na-eji enzymes dabara adaba maka ịhazi ọnụ ọgụgụ dị ukwuu nke ụdị, ọ dịkwa ọnụ ahịa nke ọma.Dị ka ndị dị otú a, enwere ogologo oge oku siri ike sitere n'aka ndị na-ahụ maka ahụike maka mmepe nke nyocha nke enzyme dị otú ahụ.Ya mere, anyị mepụtara nyocha ọhụrụ site na iji "usoro dipeptide".Kpọmkwem, anyị chọpụtara "Fructosyl-peptide Oxidase" (FPOX) nke enwere ike iji dị ka enzyme maka nyocha a.Nke a mere ka anyị nwee ihe ịga nke ọma n'inweta nke mbụ n'ụwa site n'imepụta eziokwu nke nyocha enzyme HbA1c."Usoro dipeptide" a na-eji Protease (Proteolytic enzyme) imebi HbA1c n'ime ọbara, wee tụọ ọkwa nke dipeptides saccharified nke emepụtara site na iji FPOX.Usoro a nwetara nnabata dị mma nke ukwuu n'ihi uru ya dị mfe, dị ọnụ ala na ngwa ngwa, yana reagent HbA1c na-eji FPOX abatala iji mee ihe n'ụwa niile.
