Glucose-6-phosphate dehydrogenase (G6PDH)
Nkọwa
Glucose 6 phosphate dehydrogenase (G6PD) erughi bụ ihe nketa nke mkpụrụ ndụ ọbara uhie na-agbaji (hemolysis) mgbe ahụ na-ekpughere ụfọdụ nri, ọgwụ, ọrịa ma ọ bụ nchekasị.Ọ na-eme mgbe mmadụ na-efu ma ọ bụ nwere ọkwa dị ala nke enzyme glucose-6-phosphate dehydrogenase.Enzyme a na-enyere mkpụrụ ndụ ọbara uhie aka ịrụ ọrụ nke ọma.Mgbaàmà n'oge ihe omume hemolytic nwere ike ịgụnye mmamịrị gbara ọchịchịrị, ike ọgwụgwụ, ịcha ọbara ọbara, ọnụma obi ọsọ ọsọ, mkpụmkpụ ume, na ịcha akpụkpọ ahụ (jaundice).A na-eketa ụkọ G6PD n'ụzọ nkwụghachi azụ metụtara X yana mgbaàmà na-adịkarị na ụmụ nwoke (karịsịa ndị America America na ndị si n'akụkụ ụfọdụ nke Africa, Asia, na Mediterranean).Ihe kpatara ya bụ mgbanwe mkpụrụ ndụ ihe nketa na mkpụrụ ndụ ihe nketa G6PD.
Glucose-6-Phophate Dehydrogenase (G-6-PDH) nwere akụkụ abụọ nke nha nha molekul. E bipụtala usoro amino acid nke monomer.G-6-PDH ejiriwo nyocha nyocha maka nicotinamide adenine dinucleotide na anụ ahụ pyridine. nucleotides.Enwere ike ịmegharị G-6-PDH site na ngwọta urea-denatured.
Glucose 6-phosphate dehydrogenase bụ isi ihe na-achịkwa enzyme na nzọụkwụ mbụ nke pentose phosphate ụzọ.G-6-P-DH oxidizes glucose-6-phosphate n'ihu NADP+ inye 6-phosphogluconate.Polyacrylamide gel electrophoresis, ntụpọ ọrụ, yana mgbochi yiast G-6- PDH antibody immunoblotting ọmụmụ egosila na G-6-PDH bụ glycoprotein.
Ọdịdị Chemical
Ụkpụrụ mmeghachi omume
D-glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | Ọcha amorphous ntụ ntụ, lyophilized |
| Ihe omume | ≥150U/mg |
| Ịdị Ọcha (SDS-PAGE) | ≥90% |
| Solubility (10mg ntụ ntụ / ml) | Kpochapụ |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Ụgbọ njem na nchekwa
Ụgbọ njem: Ambient
Nchekwa:Na-echekwa na 2-8°C
Nnwale akwadoroNdụ:Afọ 2
