Ribonuclease III (RNase III)
Nkọwa
Ngwaahịa a bụ ribonuclease III (RNase III) nke E. coli gosipụtara na ya.Exonuclease a kapịrị ọnụ na-agbaji RNA nwere eriri abụọ (dsRNA) wee wepụta iberibe 12-35bp dsRNA nwere 5'-PO4 na 3'-OH, 3' overhangs.
Ọdịdị Chemical
Nkọwa nkeji
Nkọwa otu ọrụ: Otu nkeji na-ezo aka na ọnụọgụ enzyme achọrọ iji weda 1 μg nke
dsRNA gaa siRNA na sistemu mmeghachi omume 50 μL na 37°C maka nkeji iri abụọ.
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Ọrụ Exonuclease | weputara 0.1% nke mkpokọta redio |
| Ọrụ DNA na-abụghị nke akọwapụtara | Enweghị ike ịhụ ya |
| Nnwale ịdị ọcha nke Protein (SDS-PAGE) | ≥ 95% |
| Ọrụ RNase (Ngbari Agbari) | 90% nke mkpụrụ RNA ka emebibeghị |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ikpụrụ kpọrọ nkụ
Nchekwa:Na-echekwa na -25 ~ -15 Celsius
Nnwale ndụ akwadoro:Afọ 2
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
