Otu nzọụkwụ RT-qPCR nyocha ngwa
Nkọwa
Otu nzọụkwụ qRT-PCR Probe Kit bụ nke ahaziri maka qPCR na-eji RNA ozugbo (dịka nje RNA) dị ka ndebiri.N'iji mkpụrụ ndụ ihe nketa kpọmkwem (GSP), ntụgharị ntụgharị na qPCR nwere ike mechaa n'otu tube, na-ebelata nke ọma usoro pipetting na ihe ize ndụ nke mmetọ.Enwere ike ịgbanyụ ya na 55 ℃ na-enweghị emetụta arụmọrụ na uche nke qRT-PCR.Na-ejikọta arụmọrụ kachasị elu nke HiScript III Reverse Transcriptase na champagne Taq DNA Polymerase na-ekpo ọkụ, yana sistemụ nchekwa kachasị mma, nchọpụta nchọpụta nke Otu Nzọụkwụ qRT-PCR Probe Kit nwere ike iru 0.1 pg nke mkpokọta RNA ma ọ bụ ihe na-erughị 10 nke ndebiri RNA. ma nwee nkwụsi ike nke thermal dị elu.Otu nzọụkwụ qRT-PCR Probe na-enye na Master Mix.Ngwakọta 5 × Otu Nzọụkwụ nwere ihe nchekwa kachasị mma yana dNTP/dUTP Mix, ma dabara adaba maka sistemu nchọpụta akọwapụtara nke ọma dabere na nyocha nke akpọrọ fluorescence (dịka TaqMan).
Usoro mmeghachi omume
Ngwa
| Ngwa | 100rxn | 1,000rns | 5,000 rxn |
| ddH2O na-enweghị RNase | 2 * 1 ml | 20ml | 100ml |
| 5 * otu nzọụkwụ mix | 600μl | 6 * 1 ml | 30ml |
| Otu nzọụkwụ enzyme mix | 150μl | 2 * 750 ml | 7.5 ml |
| 50* ROX ntụaka agba agba 1 | 60μl | 600μl | 3 * 1 ml |
| 50* ROX ntụaka agba agba 2 | 60μl | 600μl | 3 * 1 ml |
a.Nchekwa otu nzọụkwụ gụnyere dNTP Mix na Mg2+.
b.Ngwakọta Enzyme na-enwekarị azụ
transcriptase, Hot Start Taq DNA polymerase (mgbanwe mgbochi mmadụ) na RNase inhibitor.
c.Ejiri ya iji dozie njehie nke sinal fluorescene n'etiti olulu mmiri dị iche iche.
c.ROX: Ịkwesịrị ịhọrọ nhazi n'ụdị ngwaọrụ nnwale.
Ngwa
Nchọpụta nke pathogens
nchoputa na nyocha nke etuto
Nchọpụta ọrịa anụmanụ
Nchọpụta mmalite nke ọrịa nke eketa
Nchọpụta nke microorganisms pathogenic na nri
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -30 ~ -15 ℃.
Ndụ Shief:1 afọ
