Uracil DNA Glycosylase (UDG/UNG)
Nkọwa
Heat-Labile UDG (uracil DNA glycosylase) na-eme ka ntọhapụ nke uracil efu sitere na DNA nwere uracil site na hydrolyzing njikọ N-glycoside n'etiti ntọala uracil na skeleton sugar-phosphate.E jiri ya tụnyere UDG enzymes, okpomọkụ-Labile UDG nwere ike ịzenarị nbibi nke ngwaahịa njupụta dU nwere site na ọrụ fọdụrụnụ nke UDG anaghị arụ ọrụ na ụlọ okpomọkụ.Heat-Labile UDG (uracil DNA glycosylase) na-arụ ọrụ na ọnụ ụlọ okpomọkụ ma dị mfe ma dị mfe ịgbanyụ.
Ọdịdị Chemical
Nkọwapụta
| Enzyme | Glycosylase |
| Ihe nchekwa dakọtara | Nchekwa nchekwa |
| Ọkụ anaghị arụ ọrụ | 50°C, 10 nkeji |
| Nkọwa nkeji | A kọwapụtara otu nkeji (U) dị ka ọnụọgụ enzyme achọrọ iji kpata hydrolysis nke 1 μg dU nwere dsDNA n'ime nkeji 30 na 25°C. |
Ngwa
1. Ọmụmụ nke nrụzi DNA na nchọpụta mmụgharị.
2.Increase cloning arụmọrụ nke PCR ngwaahịa.
3.Mbawanye arụmọrụ nke mutagenesis na-eduzi saịtị.
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -15 ℃ ~ -25 ℃
Ndụ Shief:afọ 2
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
