Otu nzọụkwụ RT-qPCR SYBR Green Kit

Universal Blue qPCR Master Mix (Dye Based) bụ ihe ngwọta maka 2 × ezigbo oge quantitative PCR amplification ji oke uche na nkọwapụta, na-acha anụnụ anụnụ na agba, ma nwee mmetụta nke ntinye mgbakwunye sample.Ihe bụ isi akụrụngwa Taq DNA polymerase na-eji mgbochi na-ekpo ọkụ mmalite na-egbochi mmụba na-abụghị nke a kapịrị ọnụ n'ihi nkwụsị nke primer n'oge nkwadebe nlele.N'otu oge ahụ, usoro ahụ na-agbakwụnye ihe ndị na-akwalite iji melite nrụpụta arụmọrụ nke mmeghachi omume PCR ma mee ka mmụba nke mkpụrụ ndụ ihe nketa nwere ọdịnaya GC dị iche iche (30 ~ 70%), ka PCR nke ukwuu wee nweta mmekọrịta dị mma n'ahịrị n'ọtụtụ buru ibu. mpaghara.Ngwaahịa a nwere ROX Passive Reference Dye pụrụ iche, nke dabara na ngwa qPCR niile.Ọ dịghị mkpa ịgbanwe ntinye nke ROX na ngwá ọrụ dị iche iche.Ọ dị mkpa naanị ịgbakwunye primers na ndebiri iji kwadebe usoro mmeghachi omume maka mmụba.