Homocysteine (HCY)
Nkọwa
A na-eji Homocysteine (HCY) chọpụta homocysteine n'ime ọbara mmadụ.Homocysteine (Hcy) bụ amino acid nke nwere sọlfọ na-emepụta site na metabolism nke methionine.80% nke Hcy na-ejikọta na protein site na njikọ disulfide n'ime ọbara, ọ bụ naanị obere akụkụ nke homocysteine free na-ekere òkè na mgbasa.Ọkwa Hcy nwere njikọ chiri anya na ọrịa obi.bụ ihe dị mkpa dị ize ndụ maka ọrịa obi.Mmụba Hcy dị n'ọbara na-akpali mgbidi arịa ọbara ka ọ mebie arịa akwara, na-eduga na mbufụt na nhazi ọkwa n'ahụ mgbidi arịa ahụ, nke na-emecha na-ebute nkwụsị nke ọbara n'ime obi.N'ime ndị ọrịa nwere hyperhomocystinuria, mmebi mkpụrụ ndụ ihe nketa siri ike na-emetụta metabolism Hcy, na-ebute hyperhomocysteinemia.Nrụrụ mkpụrụ ndụ ihe nketa dị nro ma ọ bụ ụkọ nri nke vitamin B ga-eso ya na mmụba dị oke ma ọ bụ dị nro nke Hcy, nke ga-abawanye ohere nke ọrịa obi.Hcy dị elu nwekwara ike ịkpata ntụpọ nwa dị ka ntụpọ akwara akwara na mmebi ọmụmụ.
Ọdịdị Chemical
Ụkpụrụ ule
A na-atụgharị Hcy Oxidized ka ọ bụrụ Hcy efu, na Hcy efu na-emeghachi omume na serine n'okpuru catalysis nke CBS iji mepụta L-cystathionine.L-cystathionine na-emepụta Hcy, pyruvate na NH3 n'okpuru catalysis nke CBL.Enwere ike ịchọpụta pyruvate site na mmeghachi omume okirikiri a site na lactate dehydrogenase LDH na NADH, na ngbanwe nke NADH na NAD dabara kpọmkwem na ọdịnaya Hcy dị na nlele.
Ụgbọ njem na nchekwa
Ụgbọ njem:2-8 Celsius C
Oge nchekwa na nkwado:Ekwesịrị ịchekwa reagents na-emegheghị na 2-8 Celsius C n'ọchịchịrị, yana oge nkwado bụ ọnwa 12;Mgbe emepechara, a ga-echekwa reagents n'ọchịchịrị na 2-8 Celsius C, yana oge nkwado bụ ọnwa 1 n'okpuru ọnọdụ enweghị mmetọ;The reagents ekwesịghị oyi kpọnwụrụ.
Rịba ama
Ihe nlele chọrọ: Ihe nlele ahụ bụ ọbara ọhụrụ ma ọ bụ plasma (heparin anticoagulation, 0.1mg heparin nwere ike igbochi ọbara 1.0ml).Biko tinye plasma ozugbo ị nwetachara ọbara, ma ọ bụ tinye n'ime friji na centrifu n'ime awa 1.
