ELEISA KIT maka Trypsin

A na-ejikarị trypsin recombinant eme ihe n'ichepụta biopharmaceutical-n'oge nkwadebe cell ma ọ bụ maka mgbanwe na ịgbalite ngwaahịa.Trypsin nwere ihe egwu nchekwa ma a ga-ewepụrịrị tupu ahapụpụta ngwaahịa ikpeazụ.Ngwa Sanwichi a bụ maka nleba nke Residual Trypsin na omenala mkpụrụ ndụ na usoro ndị ọzọ na mmepụta biopharmaceutical mgbe eji Trypsin.

Ngwa a bụ Enzyme-Linked Immunosorbent Assay (ELISA).Ejikwa ọgwụ mgbochi Porcine trypsin kpuchie efere ahụ.A na-agbakwunye trypsin dị na nlele ma na-ejikọta ya na ọgwụ mgbochi ndị ekpuchiri n'olulu mmiri.Mgbe ahụ, a na-agbakwunye ọgwụ mgbochi Porcine trypsin biotinylated wee jikọta na trypsin n'ime nlele ahụ.Mgbe ịsachara, a na-agbakwunye HRP-Streptavidin ma jikọta na mgbochi biotinylated trypsin.Mgbe incubation gasịrị, a na-asachapụ HRP-Streptavidin.Mgbe ahụ, a na-agbakwunye ihe ngwọta TMB na catalyzed site na HRP iji mepụta ngwaahịa na-acha anụnụ anụnụ nke gbanwere na-acha odo odo ka gbakwunyere ngwọta nkwụsị acidic.Njupụta nke odo odo dabara na ọnụọgụ nke trypsin ebumnuche
sample e weghaara na efere.A na-atụ ihe nnabata ahụ na 450 nm.