Deoxyribonuclease I (Dnase I)

DNase I (Deoxyribonuclease I) bụ endodeoxyribonuclease nke nwere ike igbari DNA otu ma ọ bụ nwere eriri abụọ.Ọ na-amata ma kewaa njikọ phosphodiester iji mepụta monodeoxynucleotides ma ọ bụ oligodeoxynucleotides nke nwere otu ma ọ bụ okpukpu abụọ nwere otu phosphate na 5'-terminal na hydroxyl na 3'-terminal.Ọrụ nke DNase I dabere na Ca 2+ ma nwee ike ịgbalite ya site na ion metal divalent dị ka Mn 2+ na Zn 2+.5 mM Ca 2+ na-echebe enzyme site na hydrolysis.N'ebe Mg 2+ nọ, enzyme ahụ nwere ike ịmata ma kewaa saịtị ọ bụla na eriri DNA ọ bụla.N'ebe Mn 2+ nọ, enwere ike ịmata eriri abụọ nke DNA n'otu oge wee kewaa n'otu ebe ahụ iji mepụta iberibe DNA dị larịị ma ọ bụ mpempe DNA na-adọrọ adọrọ nke nwere 1-2 nucleotides na-apụta.