D-Lactate dehydrogenase (LDH)
Nkọwa
A na-eji enzyme eme ihe maka iwepụ pyruvate na mkpebi ndị na-arụ ọrụ na NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Ojiji
1.Alkaline phosphatase jikọtara ya na protein (ọgwụ mgbochi ọrịa, streptavidin wdg,) nwere ike ịmata kpọmkwem mkpụrụ ndụ lekwasịrị anya, a pụkwara iji ya mee ihe na ELISA, WB na nchọpụta histochemical;
2.Alkaline phosphatase nwere ike iji dephosphorize 5 '-terminal nke DNA ma ọ bụ RNA iji gbochie njikọ onwe;
3. DNA ma ọ bụ RNA dephosphorylated dị n'elu nwere ike ịkpọ ya site na redio phosphates (site na T4 poly-nucleotide kinase)
Ọdịdị Chemical
Usoro mmeghachi omume
D-Lactate + NAD+→ Pyruvate +NADH + H+
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | Ọcha amorphous ntụ ntụ, lyophilized |
| Ihe omume | ≥200U/mg |
| Ịdị ọcha (SDS-PAGE) | ≥90% |
| Solubility (10mg ntụ ntụ / ml) | Kpochapụ |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ambient
Nchekwa:Na-echekwa na -20°C (Ogologo oge), 2-8°C (obere oge)
Nnwale akwadoroNdụ:Afọ 2
ngwaahịa ndị metụtara ya
Dee ozi gị ebe a ziga anyị ya
