Ngwa ule Glycohemoglobin A1c (HbA1c).
Uru
● Ezi izi ezi
● Ike mgbochi nnyonye anya siri ike
● Nkwụsi ike dị mma
Ọdịdị Chemical
Ngwa
Nnwale in vitro maka ikpebi ọnụọgụ HbA1c n'ọbara mmadụ niile na sistemu fotometric.HbA1c bụ ngwaahịa hemoglobin (Hb) nke na-emepụta mmeghachi omume glycation na-adịghị ngwa ngwa na nke na-aga n'ihu n'okpuru glucose ọbara dị elu.Glucose na-agbanwe hemoglobin kpọmkwem na n-terminal valine residue iji mepụta haemoglobin glycated.N'okpuru ọnọdụ physiological nkịtị, mmepụta nke ngwaahịa mmeghachi omume glycosylation na-abụghị enzymatic dabara adaba na ntinye nke reactants.Ebe ọ bụ na mkpokọta hemoglobin ka kwụsiri ike, ọkwa glycosylation na-adaberekarị na ntinye glucose yana metụtara ogologo haemoglobin na mkpughe glucose.Ya mere, HbA1c bụ ezigbo ngosipụta maka nkezi ọkwa glucose ọbara nke ndị ọrịa n'ime ọnwa 2-3 gara aga.
Ụkpụrụ
N'okpuru ọrụ nke protease, a na-ebipụ n-terminal nke yinye β na HbA1c ma hapụ glycosylated dipeptides.Na mmeghachi omume mbụ, enwere ike nweta ntinye uche Hb site na ịlele nnabata nke 480 nm.Na mmeghachi omume nke abụọ, fructosyl peptide oxidase (FPOX) na-arụ ọrụ na glycosylated dipeptides iji mepụta hydrogen peroxide nke nwere ike meghachi omume na ndị ọrụ chromogenic iji mepụta nnabata na 660nm n'ihu peroxidase, mgbe ahụ enwere ike nweta ntinye nke HbA1c site na ịlele nnabata nke nnabata. 660nm.Dabere na mkpokọta HbA1c enwetara na ntinye Hb, enwere ike ịgbakọ pasentị HbA1c (HbA1c%).
Ọdabara
Hitachi 7180/7170/7060/7600 akpaka biochemical analyser, Abbot 16000, OLYMPUS AU640automatic biochemical analyzer
Reagents
| Ngwa | Ntinye uche |
| Reagent 1 (R1) | |
| Ihe nchekwa dị mma | 100 mmol / L |
| PRK | 500KU/L |
| DA-67 | 10 mmol / L |
| Reagents 2 (R2) | |
| Ihe nchekwa dị mma | 100 mmol / L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3 (R3) | |
| Ihe nchekwa dị mma | 100 mmol / L |
Ụgbọ njem na nchekwa
Ụgbọ njem:Ambient
Nchekwa na kwụsie ike:
Ruo ụbọchị njedebe egosiri na labelụ, mgbe echekwara na-emegheghị na 2-8 ℃ ma chebe ya pụọ na ọkụ.Ozugbo emepere ya, reagents na-akwụsi ike ruo ụbọchị 28 mgbe etinyere ya na friji na nyocha ma ọ bụ friji.
A ga-ezere mmetọ nke reagents.Akwụsịla reagents.
Ozugbo etisasịwo, calibrator na-akwụsi ike maka ụbọchị 15 na 2-8 ℃, njikwa ahụ kwụsiri ike maka ụbọchị 7 na 2-8 ℃, anaghị ajụ oyi.
Ndụ nchekwa:1 afọ
