Bst 2.0 DNA Polymerase (Glycerol efu, njupụta dị elu)
Bst DNA polymerase V2 sitere na Bacillus stearothermophilus DNA Polymerase I, nke nwere ọrụ 5′→3′ DNA polymerase na ọrụ nnọchi siri ike, mana ọ nweghị ọrụ 5′→3′ exonuclease.Bst DNA Polymerase V2 dabara nke ọma maka mbugharị strand, isothermal amplification LAMP (Agbatị isothermal na-agba agba) na usoro ngwa ngwa.Nke a Bst DNA polymerase V2 nwere ike igbochi ọrụ DNA polymerase na ụlọ okpomọkụ, nke mere na enwere ike ịrụ ọrụ ya na usoro mmeghachi omume nwere ike ịmepụta ya na ụlọ okpomọkụ, na-egbochi mmụba na-abụghị nke a kapịrị ọnụ na imeziwanye arụmọrụ nke mmeghachi omume ahụ, na nsụgharị a nwere ike. bụrụ lyophilized.Tụkwasị na nke ahụ, ọ nwere ike ịhapụ ọrụ ya na okpomọkụ dị elu, si otú a na-ewepụ mkpa maka nzọụkwụ mmalite dị iche iche.
Ngwa
| Akụkụ | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-free) (32U/μL) | 0.05 ml | 0,25 ml | 2.5 ml |
| Ihe nchekwa 10 × HC Bst V2 | 1.5 ml | 2 × 1.5 ml | 3 × 10 ml |
| MgSO4 (100mM) | 1.5 ml | 2 × 1.5 ml | 2 × 10 ml |
Ngwa
1.LAMP isothermal amplification
2.Ngwongwo DNA nke otu mmeghachi omume mbugharị
3.Usoro mkpụrụ ndụ ihe nketa GC dị elu
4.Usoro DNA nke ọkwa nanogram.
Ọnọdụ Nchekwa
Ụgbọ njem n'okpuru 0 Celsius ma debe ya na -25C ~ -15C.
Nkọwa nkeji
A kọwapụtara otu nkeji dị ka ọnụọgụ enzyme nke na-etinye 25 nmol nke dNTP n'ime ihe na-adịghị edozi acid n'ime nkeji 30 na 65°C.
Mmeghachi omume LAMP
| Ngwa | 25 μLSistemu |
| Ihe nchekwa 10 × HC Bst V2 | 2.5 μl |
| MgSO4 (100mM) | 1.5 μl |
| dNTP (10mM nke ọ bụla) | 3.5 μl |
| SYTO™ 16 Green (25×)a | 1.0 μl |
| Ngwakọta mbụb | 6 μl |
| Bst DNA Polymerase V2 (Glycerol-free) (32 U/ul) | 0.25 μl |
| Ụdị | × μL |
| ddH₂O | Ihe dị ka 25 μl |
ndetu:
1) a.SYTOTM 16 Green (25×): Dị ka mkpa nnwale si dị, a pụrụ iji agba agba ndị ọzọ dochie anya;
2) b.Ngwakọta mbụ: enwetara site na ịgwakọta 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB na mpịakọta ndị ọzọ.
Mmeghachi omume na ọnọdụ
1 × HC Bst V2 Buffer, okpomọkụ nnabata dị n'etiti 60°C na 65°C.
Ọkụ anaghị arụ ọrụ
80 Celsius C, 20 nkeji.
