2 × HiF Taq gbakwunyere Master Mix

Nọmba nwamba: HCR2014B

HIF Taq gbakwunyere Master Mix (With Dye) bụ ihe eji eme ihe 2 × premix nwere gbakwunyere HIF DNA Polymerase, dNTPs na ihe nchekwa kachasị mma.Ọgwụ mgbochi monoclonal abụọ na ụlọ okpomọkụ nke na-egbochi ọrụ polymerase na 3′→5′exonuclease ọrụ na-agbakwunyere na ngwakọta nnabata maka mfe na nke ukwuu kpọmkwem Hot Start PCR.A na-agbakwunye ihe ndọtị ahụ na ngwakọta nna ukwu iji nye enzyme ogologo ike ike nke iberi, ogologo nke mmụba nwere ike ruo 13 kb, enzyme nwere ọrụ 5′→3′ DNA polymerase na 3′→5′ ọrụ exonuclease, ntụkwasị obi ya bụ ugboro 83 nke Taq DNA polymerase, nke bụ okpukpu 9 karịa nke DNA polymerase nkịtị.Ọ dị mma maka ịkwalite ndebiri dị mgbagwoju anya, ngwaahịa nkwalite bụ njedebe na-adịghị mma.

2 × HIF Taq gbakwunyere Master Mix (With Dye) nwere uru nke ngwa ngwa ma dị mfe, nghọta dị elu, nkọwa siri ike, nkwụsi ike dị mma, wdg, usoro mmeghachi omume naanị kwesịrị ịgbakwunye primers na ndebiri, enwere ike ịbawanye ya site na abụọ- usoro nzọụkwụ, ime ka usoro nnwale dị mfe na ichekwa oge.Ngwaahịa a nwere ihe eji esiji ihe ngosi electrophoresis, yana ngwaahịa PCR enwere ike iji ya ozugbo maka electrophoresis.Na mgbakwunye, ngwaahịa ahụ nwekwara ihe nchebe pụrụ iche, nke mere na ngwakọta nna ukwu nwere ike ịnọgide na-arụ ọrụ kwụsiri ike mgbe kpochara oyi ugboro ugboro.

Ọnọdụ Nchekwa

Ekwesịrị ịchekwa ngwaahịa na -25 ~ -15 ℃ maka otu afọ.

Nkọwapụta

Ntuziaka

1.Sistemụ mmeghachi omume PCR

2.Eji ndebiri dị iche iche akwadoro

3.Protocol Amplification

1) Protocol nzọụkwụ abụọ (mgbagwoju anya template)

2) Protocol nzọụkwụ atọ (usoro oge niile)

3) Protocol gradient na-eme ka ọ ghara ịdị (nchịkọta mgbagwoju anya)

Atụmatụ n'okpuru usoro nkwalite nkwalite dị iche iche

Ihe ndetu

Biko yiri PPE dị mkpa, uwe ụlọ nyocha na uwe aka, iji hụ na ahụike na nchekwa gị!