Ultra Nuclease
UltraNuclease bụ genetically engineeredendonuclaese nke sitere na Serratia marcescens, nke nwere ike imebi DNA ma ọ bụ RNA, ma okpukpu abụọ ma ọ bụ otu eriri, linear ma ọ bụ okirikiri n'okpuru ọnọdụ dịgasị iche iche, na-eweda nucleic acid kpamkpam n'ime 5'-monophosphate oligonucleotides na ogologo 3-5base. .Mgbe mgbanwe nke mkpụrụ ndụ ihe nketa injinia, ngwaahịa e fermented, kwupụta, na ọcha na Escherichia coli (E. coli), nke na-ebelata theviscosity nke cell supernatant na cell lysate nnyocha sayensị, ma na-emeziwanye ọcha arụmọrụ na arụ ọrụ nnyocha nke protein.Enwere ike iji ya na usoro ọgwụgwọ mkpụrụ ndụ ihe nketa, nchacha nje, mmepụta ọgwụ mgbochi, protein na polysaccharide ọgwụ ụlọ ọrụ dị ka onye na-anabata ihe mkpofu nucleic acid reagent.
Njirimara ngwaahịa
| CAS Mba. | 9025-65-4 |
| EC Mba. | |
| Ibu molekụla | 30kDa |
| Ebe isoelectric | 6.85 |
| Ịdị ọcha nke Protein | ≥99% (SDS-PAGE & SEC-HPLC) |
| Ihe omume akọwapụtara | ≥1.1 × 106U/mg |
| Okpomọkụ kacha mma | 37°C |
| pH kacha mma | 8.0 |
| Ọrụ Protease | adịghị mma |
| Ibu ibu | 10CFU/100,000U |
| Protein cell ọbịa nke fọdụrụ | ≤10ppm |
| Ọla dị arọ | ≤10ppm |
| Endotoxin nke nje bacteria | 0.25EU/1000U |
| Nchekwa nchekwa | 20mM Tris-HCl, pH 8.0, 2mM mgCl2, 20mM NaCl,50% glycerol |
Ọnọdụ nchekwa
≤0°C njem;-25~-15°C Nchekwa,2 afọ ndaba (zere kefriza-thawing).
Nkọwa nkeji
Ọnụ ego nke enzyme eji agbanwe uru absorption nke △A260 site na 1.0 n'ime 30min na 37 Celsius C, pH 8.0, dakọtara na 37μg salmon sperm DNA gbarie site na ịkpụ n'ime oligonucleotides, akọwara dị ka otu na-arụ ọrụ (U).
njikwa mma
Protein cell ọbịa nke fọdụrụ: ELISA ngwa
•Mgbochi Ihe fọdụrụ: 250KU/mL UltraNuclease meghachiri omume na mkpụrụ maka 60min, ọ dịghị ọrụ ahụrụ.
•Endotoxin nke nje bacteria: LAL-Nnwale, Pharmacopoeia nke ndị mmadụ Republic of China Mpịakọta 4 (2020 Edition) gel ókè ule usoro.Iwu izugbe (1143).
•Ibu ibu: Pharmacopoeia nke ndị mmadụ Republic of China Mpịakọta 4 (Mbipụta 2020) — Ọchịagha
Iwu maka ule ịmụ nwa (1101), PRC National Standard, GB 4789.2-2016.
•Ọla dị arọ:ICP-AES, HJ776-2015.
Ọrụ
Emechibidoro ọrụ UltraNuclease nke ukwuu mgbe itinye uche SDS karịrị 0.1% ma ọ bụ EDTA
Ntinye uche karịrị 1mM.Surfactant Triton X-100, Tween 20 na Tween 80 enweghị mmetụta na nuclease.
Njirimara mgbe ịta ahụhụ dị n'okpuru 1.5%.
| Ọrụ | Ọrụ kacha mma | Ọrụ bara uru |
| Okpomọkụ | 37 ℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0-11.0 |
| mg2+ | 1-2mm | 1-15mm |
| DTT | 0-100mm | > 100mM |
| 2-Mercaptoethanol | 0-100mm | > 100mM |
| Monovalent metal ion (Na+, K+ etc.) | 0-20mm | 0-200mm |
| PO43- | 0-10mm | 0-100mm |
Ojiji na usoro onunu ogwu
• Wepụ exogenous nucleic acid na ngwaahịa ọgwụ mgbochi, belata ihe ize ndụ nke fọdụrụ n'ime nucleic acid toxicity na melite ngwaahịa nchekwa.
• Belata viscosity nke mmiri mmiri nri nke nucleic acid kpatara, belata oge nhazi ma mụbaa mkpụrụ protein.
• Wepu acid nucleic nke kechie urughuru (nje virus, inclusion body, etc.), nke na-enyere aka.
ka ntọhapụ na ọcha nke urughuru.
| Ụdị nnwale | Mmepụta Protein | Nje Virus,Vaccine | Ọgwụ Cell |
| Nọmba sel | 1 g cell mmiri arọ (weghachitere ya na ihe nchekwa 10ml) | 1L gbaa ụka mmiri mmiri supernatant | Omenala 1L |
| Opekempe usoro onunu ogwu | 250U | 100U | 100U |
| Ngwọta akwadoro | 2500U | 25000U | 5000U |
• Ọgwụgwọ nuklia nwere ike imezi mkpebi na mgbake nke ihe nlele maka chromatography kọlụm, electrophoresis na nyocha blotting.
• Na usoro ọgwụgwọ mkpụrụ ndụ ihe nketa, a na-ewepụ acid nucleic iji nweta nje ndị metụtara adeno dị ọcha.
