prou
Ngwaahịa
Otu nzọụkwụ RT-qPCR SYBR Green Premix HCB5140A Foto egosipụtara
  • Otu nzọụkwụ RT-qPCR SYBR Green Premix HCB5140A

Otu nzọụkwụ RT-qPCR SYBR Green Premix


Mpempe nwamba: HCB5140A

ngwugwu: 100RXN/1000RXN/10000RXN

Otu nzọụkwụ RT-qPCR SYBR Green Premix bụ maka ọnụọgụ fluorescence dabere na SYBR Green I dye.

Nkọwa ngwaahịa

Nkọwa ngwaahịa

Mpempe nwamba: HCB5140A

Otu nzọụkwụ RT-qPCR Syber Green Premix bụ maka ọnụọgụ fluorescence dabere na SYBR Green I dye.N'iji mkpụrụ ndụ ihe nketa kpọmkwem, ntụgharị ntụgharị na mmeghachi omume qPCR na-agwụcha n'otu tube, na-ewepụ mkpa maka imeghe okpu ugboro ugboro na ọrụ ọkpọkọ, na-eme ka arụmọrụ nyocha dịkwuo mma na ibelata ihe ize ndụ nke mmetọ.Maka nlele RNA, ngwa ahụ na-eji Reverse Transcriptase na-eguzogide okpomọkụ maka njikọ cDNA nke ọma yana HotStart Taq DNA Polymerase maka mmụba ọnụọgụ.N'okpuru sistemụ nchekwa kachasị mma, ọgụgụ isi nke ngwa ahụ nwere ike ịdị elu dị ka 0.1 pg maka ebumnuche egosipụtara nke ukwuu yana ihe ruru 1 pg maka ebumnuche egosipụtara nke ọma.Ngwa ahụ dabara maka mmụba na ọnụọgụ nke ihe nlele DNA.Ọ na-enyere aka ịchọpụta na ọnụọgụ nucleic acid sitere na ihe atụ osisi na anụmanụ dị iche iche, sel na microorganisms.


  • Nke gara aga:
  • Osote:

  • Ngwa

    No

    Aha

    Olu

    Olu

    1

    Ihe nchekwa dị elu

    250 μl

    2 × 1.25 ml

    2

    Ngwakọta Enzyme dị elu

    20 μl

    200 μl

    3

    RNase Free H2O

    250 μl

    2 × 1.25 ml

     

    Ọnọdụ Nchekwa

    Ekwesịrị ịchekwa ngwaahịa a na -25 ~ -15 ℃ pụọ na ìhè maka otu afọ.

     

     Ntuziaka

    1.Reaction nhazi usorod

    Ngwa

    Olu (μL)

    Olu (μL)

    Ntinye uche ikpeazụ

    Ihe nchekwa dị elu

    12.5

    25

    Ngwakọta Enzyme dị elu

    1

    2

    -

    Ndị na-aga n'ihu (10 μmol/L)a

    0.5

    1

    0.2 μmol / L

    Agbaghachi azụ (10 μmol/L)a

    0.5

    1

    0.2 μmol / L

    RNA Tamplateb

    X

    X

    -

    RNase Free H2Oc

    ruo 25

    ruo 50

    -

    ndetu:

    1) a.TNtinye uche nke ikpeazụ bụ 0.2 μmol/L, nke nwekwara ike gbanwee n'etiti 0.1 na 1μmol/L dị ka o kwesịrị.

    2) b.The reagent nwere mmetụta nke ukwuu, yana ngụkọta RNA na oke nke 1pg-1μg, na nnwale nke ihe nlele mmadụ gosipụtara ntinye kacha mma nke 1 pg-100 ng, na-achịkwa maka uru Ct n'ozuzu ya na oke 15-30 dị ka o kwesịrị.

    3) c.A na-atụ aro ka iji 20μL ma ọ bụ 50μL iji hụ na ịdị irè na nrụpụta nke nkwalite mkpụrụ ndụ ihe mgbaru ọsọ.

    4) d.Biko kwadebe n'oche oche dị ọcha ma jiri ndụmọdụ na-enweghị nuclease na tubes mmeghachi omume;A na-atụ aro ndụmọdụ nwere katrij nzacha.Zere mmetọ obe na mmetọ ikuku.

     

     2.Mmemme mmeghachi omume

    Nzọụkwụ okirikiri

    Okpomọkụ

    Oge

    okirikiri

    Ntugharị azụ azụ

    50 ℃a

    6mins

    1

    denaturation nke mbụ

    95 ℃

    5mins

    1

    Mmeghachi omume nkwalite

    95 ℃

    15 nkeji

    40

    60 ℃b

    30 sk

    Agba agbaze okirikiri

    Ngwa akụrụngwa

    1

    ndetu:

    1) a.Enwere ike ịhọrọ okpomọkụ ntụgharị ntụgharị n'etiti 50-55 Celsius dịka mkpa nnwale siri dị.Maka nlele DNA, enwere ike ịhapụ usoro ntụgharị ntụgharị.

    2) b.N'ọnọdụ ndị pụrụ iche enwere ike imezi okpomọkụ mgbakasị ahụ / mgbatị ahụ dịka uru Tm primer si dị, a na-atụ aro 60 Celsius.

     

    Ihe ndetu

    1. Ngwaahịa a bụ maka nyocha naanị.

    2. Biko rụọ ọrụ na uwe lab na uwe mwụfu, maka nchekwa gị.

     

    Dee ozi gị ebe a ziga anyị ya