Otu nzọụkwụ RT-qPCR nyocha ngwa
Nkọwa
Otu nzọụkwụ qRT-PCR Probe Kit bụ nke ahaziri maka qPCR na-eji RNA ozugbo (dịka nje RNA) dị ka ndebiri.N'iji mkpụrụ ndụ ihe nketa kpọmkwem (GSP), ntụgharị ntụgharị na qPCR nwere ike mechaa n'otu tube, na-ebelata nke ọma usoro pipetting na ihe ize ndụ nke mmetọ.Enwere ike ịgbanyụ ya na 55 ℃ na-enweghị emetụta arụmọrụ na uche nke qRT-PCR.Otu nzọụkwụ qRT-PCR Probe na-enye na Master Mix.Ngwakọta 5 × Otu Nzọụkwụ nwere ihe nchekwa kachasị mma yana dNTP/dUTP Mix, ma dabara adaba maka sistemu nchọpụta akọwapụtara nke ọma dabere na nyocha nke akpọrọ fluorescence (dịka TaqMan).
Ụkpụrụ bụ isi nke RT-qPCR
Nkọwapụta
| Nnwale ihe | Nkọwapụta | Nsonaazụ |
| (SDS PAGE) Ịdị Ọcha nke Enzyme Stock(SDS PAGE) | ≥95% | Gafe |
| Ọrụ Endonuclease | Achọpụtaghị | Gafe |
| Ọrụ Exodulease | Achọpụtaghị | Gafe |
| Ọrụ Rnase | Achọpụtaghị | Gafe |
| DNA fọdụrụ E.coli | 1 akwụkwọ/60 | Gafe |
| Usoro nyocha-arụ ọrụ | 90%≤110% | Gafe |
Ngwa
| Ngwa | 100rxn | 1,000rns | 5,000 rxn |
| ddH2O na-enweghị RNase | 2 * 1 ml | 20ml | 100ml |
| 5 * otu nzọụkwụ mix | 600μl | 6 * 1 ml | 30ml |
| Otu nzọụkwụ enzyme mix | 150μl | 2 * 750 ml | 7.5 ml |
| 50* ROX ntụaka agba agba 1 | 60μl | 600μl | 3 * 1 ml |
| 50* ROX ntụaka agba agba 2 | 60μl | 600μl | 3 * 1 ml |
a.Nchekwa otu nzọụkwụ gụnyere dNTP Mix na Mg2+.
b.Ngwakọta Enzyme na-enwekarị azụ
transcriptase, Hot Start Taq DNA polymerase (mgbanwe mgbochi mmadụ) na RNase inhibitor.
c.Ejiri ya iji dozie njehie nke sinal fluorescene n'etiti olulu mmiri dị iche iche.
d.ROX: Ịkwesịrị ịhọrọ nhazi n'ụdị ngwaọrụ nnwale.
Ngwa
Nchọpụta QPCR
Mbupu na Nchekwa
Mbupu:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -20 ℃.
Ndụ Shief:Ọnwa 18
