Ngwa mmịpụta DNA/RNA nje
Ngwa ahụ (HC1009B) nwere ike wepụta acid nucleic acid dị ọcha dị elu ngwa ngwa (DNA/RNA) site na ụdị mmiri dị iche iche dị ka ọbara, ọbara, plasma, na mmiri ịsa swab, na-eme ka nhazi nke ihe atụ yiri ya.Ngwa ahụ na-eji bead ndọta dabere na silicon superparamagnetic agbakwunyere pụrụ iche.N'ime usoro nchekwa pụrụ iche, nucleic acids kama protein na adịghị ọcha ndị ọzọ bụ nke njikọ hydrogen na njikọ electrostatic na-agbasa.A na-asa beads magnetik ndị gbasasịrị nucleic acid iji wepụ protein na nnu fọdụrụnụ.Mgbe ị na-eji ihe ntanye nnu dị ala, a na-ahapụ acids nucleic site na bead magnetik, iji nweta ebumnuche nke ikewa ngwa ngwa na ịdị ọcha nke acid nucleic.Usoro ọrụ niile dị mfe, ngwa ngwa, nchekwa na ịrụ ọrụ nke ọma, enwere ike iji acids nucleic enwetara ozugbo maka nnwale dị n'okpuru dị ka ntụgharị ntụgharị, PCR, qPCR, RT-PCR, RT-qPCR, usoro ọgbọ na-esote, nyocha biochip, wdg.
Ọnọdụ nchekwa
Chekwaa na 15 ~ 25 ℃, ma bufee ya na ụlọ okpomọkụ.
Ngwa
Ọbara, serum, plasma, swab eluent, anụ ahụ homogenate na ndị ọzọ.
Usoro nnwale
1. Ihe atụ nhazi
1.1 Maka nje virus n'ụdị mmiri mmiri dị ka ọbara, serum, na plasma: 300μL nke supernatant ejiri maka mmịpụta.
2.2 Maka ihe nlele swab: Tinye ihe nlele swab n'ime tubes nlele nwere ngwọta nchekwa, vortex maka 1 min, wee were 300μL supernatant maka mmịpụta.
1.3 N'ihi na nje na anụ ahụ homogenates, tissuesoak ngwọta, na gburugburu ebe obibi samples: guzo samples maka 5 -10 min, na-ewere 300μL nke supernatant maka mmịpụta.
2. Nkwadebe nke nkwadebereagent gbara agba
Wepụ reagents ndị etinyegoro na ngwa ahụ, tụgharịa ma gwakọta ọtụtụ oge ka iweghachite bead magnetik.Jiri nwayọ maa efere ahụ ka ọ mee ka reagents na bead magnetik imikpu n'ala ala olulu mmiri ahụ.Biko gosi na ntụzịaka nke efere ahụ ma jiri nlezianya kwatuo foil aluminom akara.
Δ Zere ịma jijiji mgbe ị na-akwapụ ihe nkiri akara ka ọ ghara ịwụfu mmiri.
3. Ọrụ nke akpaakangwa akpụ
3.1 Tinye 300μL nke ihe nlele na olulu mmiri dị na Ogidi 1 ma ọ bụ 7 nke efere olulu mmiri miri emi nke 96 (leba anya na ọnọdụ dị mma na-arụ ọrụ nke ọma).Olu ntinye nke sample dakọtara na 100-400 μL.
3.2 Tinye efere olulu mmiri dị omimi nke 96 n'ime ihe na-ewepụta acid nucleic.Yiri ogwe aka ndọta, ma hụ na ha kpuchiri mkpanaka ndọta ahụ nke ọma.
3.3 Tọọ mmemme dị ka ndị a maka mmịpụta akpaka:
3.4 Mgbe mmịpụta ahụ gasịrị, nyefee eluent site na Ogidi 6 ma ọ bụ 12 nke 96 miri emi nke efere (leba anya na ọnọdụ dị mma na-arụ ọrụ nke ọma) gaa na tube centrifuge na-enweghị Nuclease dị ọcha.Ọ bụrụ na ị naghị eji ya ozugbo, biko chekwaa ngwaahịa na -20 ℃.
Ihe ndetu
Maka iji naanị nyocha.Ọ bụghị maka ojiji na usoro nyocha.
1. Ngwaahịa ewepụtara bụ DNA/RNA.Ekwesịrị itinye nlebara anya pụrụ iche iji gbochie mmebi nke RNA site na RNase n'oge ọrụ.Ekwesịrị ịrara arịa na samplers eji.Tubọ niile na ndụmọdụ pipette kwesịrị ịgba ọgwụ mgbochi yana enweghị DNAse/RNase.Ndị na-arụ ọrụ kwesịrị iyi uwe na ihe mkpuchi na-enweghị ntụ ntụ.
2. Biko gụọ akwụkwọ ntuziaka nke ọma tupu ejiri ya, ma rụọ ọrụ nke ọma dịka ntuziaka ntụziaka.A ghaghị ime nhazi nlele n'oche dị ọcha ma ọ bụ kabinet nchekwa ndụ.
3. A ga-ekpochapụ usoro mmịpụta nucleic acid akpaka site na UV maka 30 min tupu na mgbe ejiri ya.
4. Enwere ike ịnwe akara nke bead magnet fọdụrụ na eluent mgbe mmịpụta ahụ gasịrị, yabụ zere ịchọọ cha cha magnet.Ọ bụrụ na a na-achọ beads magnetik, enwere ike iwepụ ya na nkwụnye ndọta.
5. Ọ bụrụ na enweghị ntụziaka pụrụ iche maka batches dị iche iche nke reagents, biko ejikọtala ha, ma hụ na a na-eji ngwa ndị ahụ n'ime oge nkwado.
6. Tụfuo nke ọma niile samples na reagent, juputara hichapụ na disinfect niile ọrụ na-ebupụta na 75% ethanol.
