Ngwa ihe nyocha nke Ultra Nuclease (ELISA) egosipụtara

Ngwa nyocha nke Ultra Nuclease (ELISA)

Nkesa Nọmba: 9025-65-4

EC.Nọmba: 3.1.30.2

Ngwugwu: 96 Ule

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Nkọwa ngwaahịa

Nkọwa

Ngwa ELISA Ultra Nuclease a bụ ájá nke ELISA nke a ga-arụ n'ụdị microplate.A na-etinye ihe nlele nwere ike ịnwe endonuclease n'ime olulu mmiri microtiter nke ejirila ihe mgbochi dị ọcha kpuchie mgbochi endonuclease.Mgbe mmanye na usoro ịsa ahụ nke ewepụrụ ihe ndị na-adịghị ejikọta ya, a na-agbakwunye ihe mgbochi enzyme-conjugated, anti-endonuclease detector antibody.Mmeghachi omume mgbochi ọrịa na-ebute nguzobe nke mgbagwoju Sanwichi nke akụkụ siri ike antibody-endonuclease-enzyme akpọrọ antibody.Mgbe nzọụkwụ ikpeazụ gachara, a na-agbakwunye ihe ngwọta nke mkpụrụ osisi na olulu mmiri ma meghachi omume, na-ebute mmepe agba.A na-atụ njupụta ngwa anya fotometrically ma dabara na nke
analyte ịta ugbu a na olulu mmiri.Enwere ike ịgbakọ mkpokọta endonuclease na nlele amaghi ama dabere na usoro ọkọlọtọ kwekọrọ.

Ọdịdị Chemical

Nkọwa nkeji

Ọnụọgụ nke enzyme eji agbanwe uru nnabata nke △A260 site na 1.0 n'ime nkeji 30.
na 37 Celsius C, pH 8.0, dakọtara na 37μg salmon sperm DNA gbazere site n'ịba n'ime oligonucleotides, kọwara dị ka ihe na-arụ ọrụ.

Ojiji na usoro onunu ogwu

• Wepụ exogenous nucleic acid na ngwaahịa ọgwụ mgbochi, belata ihe ize ndụ nke fọdụrụ n'ime nucleic acid toxicity na melite ngwaahịa nchekwa.
• Belata viscosity nke mmiri mmiri nri nke nucleic acid kpatara, belata oge nhazi na ịbawanye mkpụrụ protein.
• Wepu nucleic acid nke ọbọp urughuru (nje Virus, nsonye ahu, wdg), nke na-eme ka ntọhapụ na ọcha nke urughuru.
• Ọgwụgwọ nuklia nwere ike imezi mkpebi na mgbake nke nlele maka chromatography kọlụm, electrophoresis na nyocha blotting.
• Na usoro ọgwụgwọ mkpụrụ ndụ ihe nketa, a na-ewepụ acid nucleic iji nweta nje ndị metụtara adeno dị ọcha.