Peroxidase (isi iyi nke Horseradish) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) dịpụrụ adịpụ na mgbọrọgwụ nke horseradish (Amoracia rusticana) ma bụrụ nke otu ferroprotoporphyrin nke peroxidases.HRP na-ejikọta ngwa ngwa na hydrogen peroxide (H2O2).Ihe mgbagwoju anya [HRP-H2O2] nwere ike oxidize ụdị dị iche iche nke ndị nyere onyinye hydrogen:
Onye nyere onyinye + H2O2 → Onye nyere Oxidized + 2 H2O
HRP ga-eme ka mkpụrụ dị iche iche oxidize (lee Tebụl 1):
• Chromogenic
• Chemiluminescent (dị ka luminol ma ọ bụ isoluminol)
• Fluorogenic (dị ka tyramine, homovanillic acid, ma ọ bụ 4-hydroxyphenyl acetic acid)
HRP bụ otu yinye polypeptide nwere àkwà mmiri disulfide anọ.HRP bụ glycoprotein nke nwere 18% carbohydrate.Ngwakọta carbohydrate nwere galactose, arabinose, xylose, fucose, mannose, mannosamine, na galactosamine, dabere na isozyme a kapịrị ọnụ.

HRP bụ akara a na-ejikarị eme ihe maka immunoglobulins n'ọtụtụ ngwa immunochemistry dị iche iche, gụnyere immunoblotting, immunohistochemistry, na ELISA.Enwere ike ijikọ HRP na ọgwụ mgbochi site na ụzọ dị iche iche, gụnyere glutaraldehyde, periodate oxidation, site na njikọ disulfide, yana site na amino na thiol na-eduzi cross-linkers.HRP bụ akara kachasị amasị maka ọgwụ nje, ebe ọ bụ nke kacha nta na nke siri ike nke akara atọ kachasị ewu ewu nke enzyme (peroxidase, β-galactosidase, alkaline phosphatase) na glycosylation ya na-eduga n'ịbelata njikọ na-abụghị nke a kapịrị ọnụ.Ebipụtala nyocha nke glutaraldehyde na usoro njikọ oge.

A na-ejikwa Peroxidase maka ikpebi glucose4 na peroxides na ngwọta.Ọtụtụ akwụkwọ, 6-24 thes, 25-29 na dissertations 30-46 ekwupụtala iji P8375 mee ihe na usoro nyocha ha.