Otu nzọụkwụ RT-qPCR nyocha ngwa
Nkọwa
U+ Otu Nzọụkwụ RT-qPCR Probe Kit (Glycerol-free) bụ glycerol na-enweghị otu nzọụkwụ RT-qPCR reagent na-eji RNA dị ka ndebiri (dị ka nje RNA), nke dabara maka mmepe na imepụta ngwaahịa lyophilized.Ngwaahịa a na-ejikọta arụmọrụ dị elu nke otu nzọụkwụ raara onwe ya nye Reverse Transcriptase na champagne Taq DNA Polymerase na-ekpo ọkụ, yana ihe nchekwa kemịkalụ na-ekpochapụ nke ọma, nke nwere arụmọrụ nkwalite, nguzozi na nkọwapụta, yana dakọtara na ihicha dị iche iche. usoro.Na mgbakwunye, a na-ewebata usoro mgbochi mmetọ nke dUTP / UDG n'ime reagent, nke nwere ike ịrụ ọrụ na ọnụ ụlọ okpomọkụ, wepụ mmetụta nke mmetọ ngwaahịa amplification na qPCR, ma hụ na nsonaazụ ziri ezi.
Ụkpụrụ bụ isi nke RT-qPCR
Nkọwapụta
| Nnwale ihe | Nkọwapụta | Nsonaazụ |
| (SDS PAGE) Ịdị Ọcha nke Enzyme Stock(SDS PAGE) | ≥95% | Gafe |
| Ọrụ Endonuclease | Achọpụtaghị | Gafe |
| Ọrụ Exodulease | Achọpụtaghị | Gafe |
| Ọrụ Rnase | Achọpụtaghị | Gafe |
| DNA fọdụrụ E.coli | 1 akwụkwọ/60 | Gafe |
| Usoro nyocha-arụ ọrụ | 90%≤110% | Gafe |
Ngwa
| Ngwa | 100rxn | 1,000rns | 5,000 rxn |
| ddH2O na-enweghị RNase | 2 * 1 ml | 20ml | 100ml |
| 5 * otu nzọụkwụ mix | 600μl | 6 * 1 ml | 30ml |
| Otu nzọụkwụ enzyme mix | 150μl | 2 * 750 ml | 7.5 ml |
| 50* ROX ntụaka agba agba 1 | 60μl | 600μl | 3 * 1 ml |
| 50* ROX ntụaka agba agba 2 | 60μl | 600μl | 3 * 1 ml |
a.Nchekwa otu nzọụkwụ gụnyere dNTP Mix na Mg2+.
b.Ngwakọta Enzyme na-enwekarị azụ
transcriptase, Hot Start Taq DNA polymerase (mgbanwe mgbochi mmadụ) na RNase inhibitor.
c.Ejiri ya iji dozie njehie nke sinal fluorescene n'etiti olulu mmiri dị iche iche.
c.ROX: Ịkwesịrị ịhọrọ nhazi n'ụdị ngwaọrụ nnwale.
Ngwa
Nchọpụta QPCR
Mbupu na Nchekwa
Ụgbọ njem:Ngwunye ice
Ọnọdụ Nchekwa:Na-echekwa na -20 ℃.
Ndụ Shief:Ọnwa 18
