Multiplex qPCR Probe Premix

Mpempe nwamba: HCB5051A

TaqMan multiplex qPCR Master Mix (Dye Based) bụ ihe ngwọta maka 2 × ezigbo oge quantitative PCR amplification nke nwere nnukwu uche na nkọwapụta, nke na-acha anụnụ anụnụ na agba, ma nwee mmetụta nke mgbakwunye sample.Ngwaahịa a bụ 2× Mix tupu agwakọta reagent na-enyere aka ruo anọ fluorescent quantitative PCR mmeghachi omume na otu mmeghachi omume ọma.Ngwaahịa a nwere usoro mgbochi mkpụrụ ndụ gbanwetụrụ iji malite ịmalite enzyme Taq na-ekpo ọkụ, na-emeziwanye mmetụta mmụba na nkọwapụta.N'otu oge ahụ, ngwaahịa a emeziwanyela ihe nchekwa multi-reaction, nke nwere ike imeziwanye arụmọrụ nke mmeghachi omume ahụ ma kwalite nkwalite dị irè nke ndebiri ntinye uche dị ala.Enwere ike iji ngwaahịa a maka genotyping na multiplex quantitative analysis.

Nkọwapụta

Ọnọdụ Nchekwa

A na-ebufe ngwaahịa ahụ na ice akọrọ ma nwee ike ịchekwa ya na -25 ~ -15 ℃ maka afọ 2.

Ntuziaka

1. Mmeghachi omumeSistemu

ndetu:Gwakọta nke ọma tupu ejiri ya ka ịzenarị afụ oke site na ịma jijiji siri ike.

a.Ntinye uche nke izizi: Ngwakọta Primer nwere otutu ụzọ abụọ nke primers, na-abụkarị primer ọ bụla na ntinye ikpeazụ nke 0.2 μmol/L yana enwere ike ịhazi ya n'etiti 0.1 na 0.5 μmol/L dị ka o kwesịrị.

b.Nleba anya nyocha: Ngwakọta nyocha nwere ọtụtụ nyocha nwere akara ngosi fluorescence dị iche iche, yana itinye uche nke nyocha ọ bụla nwere ike gbanwee n'etiti 50 na 250 nmol/L dịka ọnọdụ akọwapụtara.

1.Rox dye reference:A na-eji ya na ngwa ngwa PCR nke adịgboroja dị ka Applied Biosystems iji mezie njehie nke mgbaàmà fluorescence na-emepụta n'etiti olulu mmiri;Ngwaahịa a enweghị ntụaka agba agba Rox.A na-atụ aro Cas # 10200 ma ọ bụrụ na achọrọ ya.

2.Ntugharị ndebiri: qPCR nwere mmetụta nke ukwuu, a na-atụ aro ka ị mebie ndebiri maka ojiji.Ọ bụrụ na template bụ cDNA ngwaahịa ngwọta, ndebiri olu ekwesịghị gafere 1/10 nke mkpokọta olu.

3.Usoro mmeghachi omume: 25μL, 30μL ma ọ bụ 50 μL ka akwadoro iji hụ na arụmọrụ na nkwughachi nke nkwalite mkpụrụ ndụ ihe mgbaru ọsọ.

4.Nkwadebe usoro: Biko kwadebe na bench dị oke ọcha, ma jiri ndụmọdụ na tubes mmeghachi omume na-enweghị ihe fọdụrụnụ nuclease;a na-atụ aro ka iji ndụmọdụ na katrij nzacha.Zere mmetọ obe na mmetọ ikuku.

2.Mmemme mmeghachi omume

ndetu:

1.Mwepu/Mgbatị: Enwere ike ịhazi ọnọdụ okpomọkụ na oge nke ọma dịka uru Tm primer emebere siri dị.

2.Nnweta mgbaàmà fluorescence: Oge nnweta mgbama fluorescence chọrọ maka ngwa nchọpụta qPCR dị iche iche, biko tọọ dabere na oke oge kacha nta.Edebere oge ọtụtụ ngwa ngwa dị ka ndị a:

20 sk: Ngwa Biosystem 7700, 7900HT, 7500 Ọsọ

31 sk: Ngwa biosystem 7300 etinyere

32 sk: Ngwa biosystems etinyere 7500

Ihe ndetu

Biko yiri PPE dị mkpa, uwe ụlọ nyocha na uwe aka, iji hụ na ahụike na nchekwa gị!