Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase bụ mmalite DNA polymerase na-ekpo ọkụ na-egbochi okpukpu abụọ site na mgbochi abụọ. Ngwaahịa a abụghị nanị na-egbochi ọrụ 5′→3′ polymerase nke Taq DNA polymerase, kamakwa na-egbochi ọrụ 5′→3′exonuclease.Ịkpọ oku maka 30 sekọnd na pre-denaturation okpomọkụ nwere ike mebie kpamkpam mgbochi na hapụ DNA polymerase ọrụ na exonuclease ọrụ.Njirimara mgbochi okpukpu abụọ nwere ike ọ bụghị naanị igbochi mmụba na-enweghị isi nke kpatara mismatch ma ọ bụ primer dimer, kamakwa nke ọma na-egbochi mbelata nke mgbaàmà fluorescence kpatara mbibi nyocha, iji mee ka nchọpụta in vitro reagent kwụsie ike n'oge njem ma ọ bụ jiri ya n'ime ụlọ. okpomọkụ.
Ngwa
| Akụkụ | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| DNA Polymerase(5 U/μL) | 50 μl | 200 μl | 2 ml | 5 ml |
Ọnọdụ Nchekwa
A na-ebufe ngwaahịa a na ice akọrọ ma nwee ike ịchekwa ya na -25 ° C ~ -15 ° C maka afọ 2.
Nkọwapụta
| Polymerase | DNA Polymerase |
| Ịdị ọcha | ≥ 95% (SDS-PAGE) |
| Mmalite na-ekpo ọkụ | Mmalite Hot arụnyere n'ime ya |
| Ọsọ mmeghachi omume | Ọkọlọtọ |
| Ọrụ Exonuclease | 5′′ 3′′ |
Ntuziaka
Ntọala mmeghachi omume
| Ngwa | Olu (μL) | Ntụkwasị obi ikpeazụ |
| 2 × nchekwaa | 25 | 1× |
| Ngwakọta primer/nyochab | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Ụdị DNAc | × | 0.1-100 n'arọ |
| ddH2O | Ruo 50 | - |
ndetu:
1) Dị ka ngwa nnwale a kapịrị ọnụ si dị, ọ dị mkpa iji dozie ihe nchekwa mmeghachi omume kwekọrọ.
2) Ọnụ ọgụgụ nke DNA na ntinye nke nyocha ma ọ bụ primers na-atụ aro ntinye uche.Enwere ike ịhazi nhazi kachasị mma dịka ọnọdụ nnwale akọwapụtara.
Usoro ịgba ígwè okpomọkụ
| Nzọụkwụ | Okpomọkụ(Celsius C) | Oge | okirikiri |
| Tupu denaturation | 95 ℃ | 5mins | 1 |
| Denaturation | 95 ℃ | 15 nkeji | 45 |
| Mgbakwunye / ndọtị | 60 ℃a | 30 skb |
ndetu:
1) A na-emezi okpomọkụ mmeghachi omume dịka uru Tm nke primers emebere.
2) Ngwa qPCR dị iche iche chọrọ oge nnweta mgbaàmà fluorescence dị iche iche, biko tọọ dị ka oge kacha dị mkpụmkpụ.
Ihe ndetu
Biko yiri PPE dị mkpa, uwe ụlọ nyocha na uwe aka, iji hụ na ahụike na nchekwa gị!
