Hexokinase (HK)
Nkọwa
Jiri Hexokinase maka mkpebi nke D-glucose, D-fructose, na D-sorbitol na nri ma ọ bụ ihe nyocha nke ndu.A na-ejikwa enzyme ahụ maka nyocha nke saccharide ndị ọzọ nke na-agbanwe na glucose ma ọ bụ fructose, ya mere ọ bara uru na nyocha nke ọtụtụ glycosides.
Ọ bụrụ na ejiri Hexokinase na-ejikọta ya na glucose-6-phosphate dehydrogenase (G6P-DH)* (nyocha glucose6-phosphate nke Hexokinase mebere), ihe nlele ekwesịghị ịbụ nke nnukwu phosphate n'ihi na G6P-DH na-egbochi phosphate nke ọma.
Ọdịdị Chemical
Ụkpụrụ mmeghachi omume
D-Hexose + ATP --mg2+→ D-Hexose-6-phosphate + ADP
Nkọwapụta
| Nnwale ihe | Nkọwapụta |
| Nkọwa | Na-acha ọcha ruo obere edo edo amorphous ntụ ntụ, lyophilized |
| Ihe omume | ≥30U/mg |
| Ịdị ọcha (SDS-PAGE) | ≥90% |
| Solubility (10mg ntụ ntụ / ml) | Kpochapụ |
| Protease | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Ụgbọ njem na nchekwa
Ụgbọ njem: Ambient
Nchekwa:Na-echekwa na -20°C (Ogologo oge), 2-8°C (obere oge)
Nnwale akwadoroNdụ:1 afọ
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