Poly A Carrier RNA

Nọmba nwamba: HC4001A

Poly A, polyadenylate, bụ ngwakọta nke 100 ~ 10000 polyadenylates, nke a na-polymerized site polynucleotide phosphorylase in vitro.Na vivo, a na-agbakwunye poly (a) na 3-terminal nke mRNA site na enzyme iji meziwanye nkwụsi ike nke mRNA.Na ntinye nke mmịpụta nucleic acid, ịgbakwunye poly A na lysate ma ọ bụ ihe ngwọta nwere ike imezi mkpụrụ nke DNA na RNA.UsoroPoly A na-eme ka mkpụrụ nke nucleic acid dị ka ndị a:

1. Saturated kọntaktị na elu adsorption nke isiokwu.Ọtụtụ akwụkwọ polypropylene nwere ọkụ eletrik kwụ ọtọ n'elu, nke ga-agbakọta acid nucleic.Onye na-ebu RNA nwere ike imeju ihe ndị ammetụta adsorption ma belata mfu nke acid nucleic lekwasịrị anya.

2. Inactivate trace nucleases: E nwere dị iche iche nucleases na ndu samples nagburugburu ebe obibi.Poly A nwere ike ime ka trace nukleases na mmịpụta ma ọ bụ ichekwa usoro ijimeziwanye mkpụrụ na nkwụsi ike nke acid nucleic ezubere iche.

3. Coprecipitation: Na nucleic acid na-adị ọcha nzọụkwụ nke mmanya mediated ọdịda mmiri ma ọ bụ na-ekekọta, poly A nwere ike coprecipitate na lekwasịrị nucleic acid ma ọ bụ na-etolite polymer ahụ.melite mgbake.

Usoro ojiji

Were ntụ ntụ lyophilized kwesịrị ekwesị, tinye DEPC mmiri a na-edozi ma ọ bụ ngwọta nnu guanidine nagbazee ya n'ime 0.1-1ug/ul, wee kechie ya ma chekwaa ya na -20 Celsius.

Ngwa

1.Virus DNA / RNA mmịpụta: na-agbakwunye 1-5ug Carrier RNA na lysate nwere ike imezi mkpụrụ nke RNA / DNA, mee ka acid nucleic lekwasịrị anya ma zere mmebi nke nucleic acid dị ọcha n'oge nchekwa.

2. Na mmịpụta micro DNA/RNA site na usoro akpụkpọ anụ kọlụm (<1ug), ịgbakwunye RNA na-ebu ibu na 1-5ug na-eme ka mkpụrụ nke nucleic acid dịkwuo mma.

3. N'ime mmanya na-egbu egbu nucleic acid na ntinye uche, mgbakwunye nke 1-2ug ụgbọelu RNA na-enyere aka melite mgbake nke obere akụkụ RNA.

4. Na quantitative probe PCR mmeghachi omume mmeghachi omume, na-agbakwunye 10-100ng ụgbọelu RNA na mmeghachi omume mmeghachi omume na-enyere aka melite uche ma belata C._Turu.